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  4. Pollen allergen Phl p 5b 蛋白, Phleum pratense (His-SUMO)

Pollen allergen Phl p 5b 蛋白, Phleum pratense (His-SUMO)

目录号: HY-P71554
产品使用指南

花粉过敏原 Phl p 5b 蛋白具有核糖核酸酶活性，推测参与宿主与病原体的相互作用。其结构构型涉及通过二硫键连接的同二聚体，突出了其在细胞过程中的分子排列。Pollen allergen Phl p 5b 蛋白, Phleum pratense (His-SUMO) 是重组的 花粉过敏原 Phl p 5b 蛋白，由 E. coli 表达，带有 N-His, N-SUMO 标签。Pollen allergen Phl p 5b 蛋白, Phleum pratense (His-SUMO) 全长 265 个氨基酸，分子量约为 ~42.1 kDa。

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描述

Pollen allergen Phl p 5b protein, with ribonuclease activity, is speculated to participate in host-pathogen interactions. Its structural configuration involves a homodimer linked by disulfide bonds, highlighting its molecular arrangement in cellular processes. Pollen allergen Phl p 5b Protein, Phleum pratense (His-SUMO) is the recombinant Pollen allergen Phl p 5b protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Pollen allergen Phl p 5b Protein, Phleum pratense (His-SUMO) is 265 a.a., with molecular weight of ~42.1 kDa.
研究背景

花粉过敏原 Phl p 5b 蛋白表现出核糖核酸酶活性，推测在宿主-病原体相互作用中发挥作用。在结构上，它形成通过二硫键连接的同二聚体，强调其在细胞过程中的分子排列。
种属

Others
表达系统

E. coli
标签

N-His;N-SUMO
蛋白编号

Q40963 (20A-284V)
基因 ID

/
蛋白结构
N-term
6*His-SUMO
Phl p 5b (20A-284V)
Accession # Q40963
C-term
同用名
Pollen allergen Phl p 5b; Allergen Phl p Vb; allergen Phl p 5b; Fragment
氨基酸序列

ADAGYAPATPAAAGAAAGKATTEEQKLIEDINVGFKAAVAAAASVPAADKFKTFEAAFTSSSKAAAAKAPGLVPKLDAAYSVAYKAAVGATPEAKFDSFVASLTEALRVIAGALEVHAVKPVTEEPGMAKIPAGELQIIDKIDAAFKVAATAAATAPADDKFTVFEAAFNKAIKESTGGAYDTYKCIPSLEAAVKQAYAATVAAAPQVKYAVFEAALTKAITAMSEVQKVSQPATGAATVAAGAATTAAGAASGAATVAAGGYKV

分子量

Approximately 42.1 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Pollen allergen Phl p 5b Protein, Phleum pratense (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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