1. 重组蛋白
  2. Others
  3. PRAK 蛋白, Human (sf9, Strep)

PRAK 蛋白, Human (sf9, Strep)

目录号: HY-P703736
产品使用指南 技术支持

PRAK/MAPKAPK5 蛋白是一种丝氨酸/苏氨酸激酶,对细胞应激和促炎细胞因子做出反应,并通过 MAP 激酶(包括 MAPK1/ERK、MAPK14/p38-alpha 和 MAPK11/p38-beta)激活肿瘤抑制因子磷酸化。最初位于细胞核,激活后,它移动到细胞质并磷酸化热休克蛋白 HSP27。PRAK 蛋白, Human (sf9, Strep) 是重组的 PRAK 蛋白,由 Sf9 insect cells 表达,带有 C-Strep 标签。

MCE 的所有产品仅用作科学研究或药证申报,我们不为任何个人用途提供产品和服务

SPR 检测服务   重组蛋白定制服务

1.  客户无需承担相应的运输费用。

2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 是否有货
50 μg   询价  
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

PRAK/MAPKAPK5 protein, a serine/threonine kinase, responds to cellular stress and pro-inflammatory cytokines and acts as a tumor suppressor through MAP kinases (including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta) activated by phosphorylation. Initially located in the nucleus, upon activation, it moves to the cytoplasm and phosphorylates the heat shock protein HSP27. PRAK Protein, Human (sf9, Strep) is the recombinant human-derived PRAK protein, expressed by Sf9 insect cells, with C-Strep labeled tag.

研究背景

PRAK/MAPKAPK5 蛋白属于丝氨酸/苏氨酸激酶家族,具有肿瘤抑制因子的功能,并通过 MAP 激酶(包括 MAPK1/ERK、MAPK14/p38-alpha 和 MAPK11)磷酸化而被激活,从而对细胞应激和促炎细胞因子做出反应/p38-beta。虽然最初位于细胞核,但在磷酸化和激活后,它转移到细胞质。该激酶的一个值得注意的靶标是热休克蛋白 HSP27,可在生理相关位点将其磷酸化。该基因显示出两种编码不同亚型的选择性剪接转录物变体。PRAK/MAPKAPK5 表现出无处不在的表达,在小肠 (RPKM 3.1)、结肠 (RPKM 2.9) 和 25 个其他组织中具有可辨别的水平,强调其广泛参与多个器官的不同生理环境。

种属

Human

表达系统

Sf9 insect cells

标签

C-Strep

蛋白编号

NP_003659 (M1-Q471)

蛋白长度

Full Length

中文名
PRAK 蛋白
预测分子量
55.6 kDa
分子量

Approximately 35-45 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

纯度

Greater than 90% as determined by reducing SDS-PAGE.;Greater than 95% as determined by reducing HPLC.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

PRAK Protein, Human (sf9, Strep) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

您最近查看的产品:

Your information is safe with us. * Required Fields.

   产品名称:

 

* 需求量:

* 客户姓名:

 

* Email:

* 电话:

 

* 公司或机构名称:

   留言给我们:

Bulk Inquiry

Inquiry Information

产品名称:
PRAK Protein, Human (sf9, Strep)
目录号:
HY-P703736
需求量: