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  4. PRG3 蛋白, Human (HEK293, His)

PRG3 蛋白, Human (HEK293, His)

目录号: HY-P71233
产品使用指南

PRG3 蛋白是一种抗压性的细胞外基质结构成分。PRG3 蛋白 可刺激粒细胞活化、组胺合成及基因表达的调控。PRG3 蛋白, Human (HEK293, His) 是重组的 PRG3 蛋白，由 HEK293 表达，带有 C-6*His 标签。PRG3 蛋白, Human (HEK293, His) 全长 208 个氨基酸，分子量约为 ~27.0 kDa。

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规格 是否有货
50 μg   询价  
100 μg   询价  

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描述

PRG3 Protein is a stress-resistant extracellular matrix structural component. PRG3 Protein can stimulate granulocyte activation, histamine synthesis and regulation of gene expression. PRG3 Protein, Human (HEK293, His) is the recombinant human-derived PRG3 protein, expressed by HEK293 , with C-6*His labeled tag. The total length of PRG3 Protein, Human (HEK293, His) is 208 a.a., with molecular weight of ~27.0 kDa.
研究背景

Proteoglycan 3 是一种抗压性的细胞外基质结构成分，与 PRG2/MBP 有相似的细胞毒性和细胞刺激活性。PRG3 体外刺激嗜碱性粒细胞超氧化物生成和 IL8 释放，以及组胺和白三烯 C4 释放。
种属

Human
表达系统

HEK293
标签

C-6*His
蛋白编号

AAI13412.1 (L18-F225)
基因 ID
蛋白结构
N-term
PRG3 (L18-F225)
Accession # AAI13412.1
6*His
C-term
同用名
Proteoglycan 3; Eosinophil Major Basic Protein Homolog; Prepro-Major Basic Protein Homolog; Prepro-MBPH; PRG3; MBPH
氨基酸序列

LHLENDAPHLESLETQADLGQDLDSSKEQERDLALTEEVIQAEGEEVKASACQDNFEDEEAMESDPAALDKDFQCPREEDIVEVQGSPRCKTCRYLLVRTPKTFAEAQNVCSRCYGGNLVSIHDFNFNYRIQCCTSTVNQAQVWIGGNLRGWFLWKRFCWTDGSHWNFAYWSPGQPGNGQGSCVALCTKGGYWRRAQCDKQLPFVCSF

分子量

Approximately 27.0 kDa
纯度

Greater than 95% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

PRG3 Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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