PRKCA 蛋白, Human (sf9, GST)

PRKCA Protein, Human (sf9, GST) is the recombinant human-derived PRKCA, expressed by Sf9 insect cells , with N-GST labeled tag. ,

PKC alpha 是一种钙激活、依赖磷脂和二酰基甘油 (DAG) 的丝氨酸/苏氨酸蛋白激酶，通过直接磷酸化 RAF1、BCL2、CSPG4、TNNT2/CTNT 等靶点或激活涉及 MAPK1/3 (ERK1/2) 和 RAP1GAP 的信号级联，参与细胞增殖、凋亡、分化、迁移、粘附、肿瘤发生、心肌肥大、血管生成、血小板功能和炎症的正负调控。它在神经胶质瘤细胞中通过磷酸化激活 RAF1 或上调 CDKN1A 促进细胞生长，同时在 PMA 刺激的肠细胞中触发细胞周期阻滞程序。PKC alpha 在巨噬细胞分化中易位至细胞核，参与伤口愈合时的粘附调节，并通过磷酸化 CSPG4 增强细胞运动性。在多种癌细胞中高表达，可作为肿瘤促进因子，并参与心肌收缩力的负调控及血管生成、血小板聚集的正调控。此外，它通过磷酸化心脏肌钙蛋白 T (TNNT2/CTNT) 调节心肌细胞功能，并在血管生成中调控内皮细胞迁移和 VEGFA 信号。在血小板中，它通过 CD36/GP4 受体和 RAP1GAP 通路激活 ITGA2B-ITGB3 整合素。PKC alpha 还参与 LPS 诱导的巨噬细胞功能调控，并通过磷酸化 EIF4G1、KIT、ATF2 等靶点影响翻译和转录。类似功能 (By similarity) 包括磷酸化 SOCS2 促进其降解，以及在血管紧张素 II 信号中磷酸化 KLHL3 以调节 WNK4 相互作用。最新研究发现 (PubMed:36040231)，它还磷酸化并激活 LRRK1，进而调控细胞内运输相关的 RAB 蛋白。

The activity of PKCα is verified by the ADP-Glo method: Incubate PKCα protein, ATP, and the substrate at room temperature for 40 minutes, then add the ADP-Glo reagent and read the luminescence signal using the chemiluminescence module of the microplate reader.

Human

Sf9 insect cells

N-GST

P17252 (A2-V672)

5578

Full Length of Mature Protein

103.2 kDa

Greater than 90% as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M trehalose, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.