PRKCD 蛋白, Human (sf9, GST)

PRKCD Protein, Human (sf9, GST) is the recombinant human-derived PRKCD, expressed by Sf9 insect cells , with N-GST labeled tag. ,

PKC δ 是一种不依赖于钙、磷脂和二酰甘油 (DAG) 依赖性的丝氨酸/苏氨酸蛋白激酶，在细胞死亡和存活中发挥双重作用，在 DNA 损伤诱导的细胞凋亡过程中充当促凋亡蛋白，并在 DNA 损伤诱导的细胞凋亡过程中充当抗凋亡蛋白。细胞因子受体引发的细胞死亡过程中的凋亡蛋白。它与肿瘤抑制有关，是 NADPH 氧化酶产生氧自由基所必需的，并且在血小板反应中充当正向或负向调节剂。PKC δ 负向调节 B 细胞增殖，在自身抗原诱导的 B 细胞耐受诱导中发挥关键作用。在各种细胞应激下，PKC δ 激活导致细胞凋亡的信号通路，包括激活促死亡转录因子 BCLAF1/Btf、调节 CHUK/IKKA-p53 轴以及与 ABL1 形成复合物。此外，它还调节细胞周期进程，介导G1/S期细胞周期进程的抑制，并激活G2/M DNA损伤检查点。PKC δ 还参与细胞周期进程的控制和保护神经胶质瘤细胞免受 TNFSF10/TRAIL 诱导的细胞凋亡。此外，它还参与抗真菌免疫并调节中性粒细胞和嗜酸性粒细胞中的 NADPH 氧化酶活性。PKC δ 在血小板功能中发挥着关键作用，充当丝状伪足形成的负调节因子并调节特定受体下游的颗粒分泌。其广泛的磷酸化靶标，如 MUC1、ELAVL1、线粒体磷脂扰乱酶 3 (PLSCR3) 和 SMPD1，强调了其在调节多种细胞过程中的多功能性。

The activity was measured by off-chip mobility shift assay(MSA). The enzyme was incubated with fluorecence-labeled substrate and Mg(or Mn)/ATP. The phosphorylated and unphosphorylated substrates were separated and detected by MSA device. The Km of PKCδ was 154.7 µM.

Human

Sf9 insect cells

N-GST

Q05655-1 (A2-D676)

5580

Partial

APFLRIAFNSYELGSLQAEDEANQPFCAVKMKEALSTERGKTLVQKKPTMYPEWKSTFDAHIYEGRVIQIVLMRAAEEPVSEVTVGVSVLAERCKKNNGKAEFWLDLQPQAKVLMSVQYFLEDVDCKQSMRSEDEAKFPTMNRRGAIKQAKIHYIKNHEFIATFFGQPTFCSVCKDFVWGLNKQGYKCRQCNAAIHKKCIDKIIGRCTGTAANSRDTIFQKERFNIDMPHRFKVHNYMSPTFCDHCGSLLWGLVKQGLKCEDCGMNVHHKCREKVANLCGINQKLLAEALNQVTQRASRRSDSASSEPVGIYQGFEKKTGVAGEDMQDNSGTYGKIWEGSSKCNINNFIFHKVLGKGSFGKVLLGELKGRGEYFAIKALKKDVVLIDDDVECTMVEKRVLTLAAENPFLTHLICTFQTKDHLFFVMEFLNGGDLMYHIQDKGRFELYRATFYAAEIMCGLQFLHSKGIIYRDLKLDNVLLDRDGHIKIADFGMCKENIFGESRASTFCGTPDYIAPEILQGLKYTFSVDWWSFGVLLYEMLIGQSPFHGDDEDELFESIRVDTPHYPRWITKESKDILEKLFEREPTKRLGVTGNIKIHPFFKTINWTLLEKRRLEPPFRPKVKSPRDYSNFDQEFLNEKARLSYSDKNLIDSMDQSAFAGFSFVNPKFEHLLED

Approximately 104.0 KDa.

Greater than 80 % as determined by reducing SDS-PAGE.

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 5% glycerol, 5 mM DTT, 0.1 M trehalose.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.