1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. Prolactin
  5. Prolactin 蛋白, Human (SF9, His)

Prolactin 蛋白, Human (SF9, His)

目录号: HY-P73380
COA 产品使用指南

催乳素蛋白作用于乳腺,通过与受体 PRLR 相互作用促进泌乳。Prolactin 蛋白, Human (SF9, His) 是重组的 催乳素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。Prolactin 蛋白, Human (SF9, His) 全长 199 个氨基酸,分子量约为 ~24.3 kDa。

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规格 价格 是否有货 数量
50 μg ¥1800 In-stock
100 μg   询价  

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描述

Prolactin Protein acts on the mammary gland, promoting lactation through its interaction with the receptor PRLR. Prolactin Protein, Human (SF9, His) is the recombinant human-derived Prolactin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Prolactin Protein, Human (SF9, His) is 199 a.a., with molecular weight of ~24.3 kDa.

研究背景

催乳素是一种主要通过促进泌乳作用于乳腺的蛋白质。它通过与其受体 PRLR 相互作用来实现这一点。

生物活性

Measured by its ability to promote proliferation of INS-1 cells and the ED50 is typically 5-120 ng/mL.

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P01236 (L29-C227)

基因 ID
蛋白结构
N-term
Prolactin (L29-C227)
Accession # P01236
His
C-term
同用名
PRL; Prolactin
氨基酸序列

LPICPGGAARCQVTLRDLFDRAVVLSHYIHNLSSEMFSEFDKRYTHGRGFITKAINSCHTSSLATPEDKEQAQQMNQKDFLSLIVSILRSWNEPLYHLVTEVRGMQEAPEAILSKAVEIEEQTKRLLEGMELIVSQVHPETKENEIYPVWSGLPSLQMADEESRLSAYYNLLHCLRRDSHKIDNYLKLLKCRIIHNNNC

分子量

Approximately 24.3 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% Glycerol, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Prolactin Protein, Human (SF9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Prolactin Protein, Human (SF9, His)
目录号:
HY-P73380
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