1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. Prolactin
  5. Prolactin 蛋白, Mouse (Sf9, His)

Prolactin 蛋白, Mouse (Sf9, His)

目录号: HY-P73381
COA 产品使用指南

催乳素蛋白表现出激素和受体结合活性。它调节乳腺发育、母性行为和 JAK-STAT 信号传导。Prolactin 蛋白, Mouse (Sf9, His) 是重组的 催乳素蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。Prolactin 蛋白, Mouse (Sf9, His) 全长 197 个氨基酸,分子量约为 ~26 kDa。

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描述

Prolactin proteins exhibit hormone and receptor binding activities. It regulates mammary gland development, maternal behavior, and JAK-STAT signaling. Prolactin Protein, Mouse (Sf9, His) is the recombinant mouse-derived Prolactin protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of Prolactin Protein, Mouse (Sf9, His) is 197 a.a., with molecular weight of ~26 kDa.

研究背景

催乳素是一种多功能蛋白质,预计具有激素活性和催乳素受体结合活性。它在各种过程的上游发挥着关键作用,包括乳腺发育、母体行为以及通过 JAK-STAT 对受体信号通路的正向调节。催乳素存在于分泌颗粒中,在海马体、垂体、胎盘和滋养层巨细胞等关键结构中表达。该基因的人类直系同源物 PRL(催乳素)对颈动脉疾病具有影响,表明其可能参与生理和病理背景。对催乳素功能的研究可能有助于更深入地了解其不同作用及其对健康的影响。

生物活性

Measured by its ability to promote proliferation of INS-1 cells and the ED50 is typically 50-500 ng/mL.

种属

Mouse

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

NP_035294.2 (L32-C228)

基因 ID

19109  [NCBI]

蛋白结构
N-term
Prolactin (L32-C228)
Accession # NP_035294.2
His
C-term
同用名
PRL; Prolactin
分子量

Approximately 26 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

溶液.

组分

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

N/A.

保存条件 & 期限

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

Prolactin Protein, Mouse (Sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Prolactin Protein, Mouse (Sf9, His)
目录号:
HY-P73381
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