蛋白 L1/VACWR088, Vaccinia virus (Sf9, His, myc)

Protein L1/VACWR088 is a crucial part of the entry fusion complex (EFC) comprising 11 proteins. It facilitates the entry of the virion core into the host cytoplasm through a two-step process involving lipid mixing and pore formation. All EFC proteins, except OPG095/L1 and OPG053/F9, contribute to the assembly or stability of the complex. Protein L1/VACWR088, Vaccinia virus (Sf9, His, myc) is the recombinant Virus-derived protein L1/VACWR088, expressed by Sf9 insect cells , with N-His, C-Myc labeled tag. The total length of Protein L1/VACWR088, Vaccinia virus (Sf9, His, myc) is 182 a.a., with molecular weight of ~26 kDa.

蛋白质 L1/VACWR088 是入口融合复合物 (EFC) 的重要组成部分，EFC 是由 11 种蛋白质组成的基本组装体。在细胞感染过程中，该复合物在促进病毒粒子核心进入宿主细胞质中发挥着关键作用，其通过两步机制涉及病毒和细胞膜的脂质混合，然后形成孔。EFC 由其组成蛋白（即 OPG053/F9、OPG076/O3、OPG086/G3、OPG094/G9、OPG095/L1、OPG099/L5、OPG107/H2、OPG143/A16、OPG104/J5、 OPG147/A21 和 OPG155/A28。值得注意的是，除了 OPG095/L1 和 OPG053/F9 之外，EFC 中的每种蛋白质对于复合物的组装或稳定性都是不可或缺的。

Virus

Sf9 insect cells

N-His;C-Myc

P07612 (G2-G183)

3707544  [NCBI]

GAAASIQTTVNTLSERISSKLEQEANASAQTKCDIEIGNFYIRQNHGCNLTVKNMCSADADAQLDAVLSAATETYSGLTPEQKAYVPAMFTAALNIQTSVNTVVRDFENYVKQTCNSSAVVDNKLKIQNVIIDECYGAPGSPTNLEFINTGSSKGNCAIKALMQLTTKATTQIAPKQVAGTG

Approximately 26 kDa

• 
  Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 3% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.