PSD95 蛋白, Rat (His)

PSD95 protein plays a crucial role in PDZ domain binding, enzyme binding and signaling receptor binding. It is a structural component of the postsynaptic density and is involved in the regulation of synaptic transmission and cellular organization. PSD95 Protein, Rat (His) is the recombinant rat-derived PSD95 protein, expressed by E. coli , with N-6*His labeled tag. The total length of PSD95 Protein, Rat (His) is 724 a.a., with molecular weight of 100-110 kDa.

PSD95 蛋白在多种功能中发挥着至关重要的作用，包括 PDZ 结构域结合、酶结合和信号受体结合。它作为突触后密度的结构成分，参与化学突触传递的调节、细胞成分组织的正向调节和突触后组织等过程。它位于不同的细胞成分中，包括树突、轴突旁节区和突触后密度。PSD95 蛋白在谷氨酸能突触中具有活性，并作为突触后密度膜的锚定成分。它与突触后膜共定位，并被确定为帕金森病和颞叶癫痫的生物标志物。在人类中，直系同源 DLG4 基因的突变与常染色体显性非综合征性智力障碍有关。[由基因组资源联盟提供，2022 年 4 月] PSD95 蛋白在大脑 (RPKM 195.0)、肾上腺 (RPKM 29.1) 和七个其他组织中表现出偏向表达。

Rat

E. coli

N-6*His

NP_062567 (M1-L724)

29495  [NCBI]

MDCLCIVTTKKYRYQDEDTPPLEHSPAHLPNQANSPPVIVNTDTLEAPGYELQVNGTEGEMEYEEITLERGNSGLGFSIAGGTDNPHIGDDPSIFITKIIPGGAAAQDGRLRVNDSILFVNEVDVREVTHSAAVEALKEAGSIVRLYVMRRKPPAEKVMEIKLIKGPKGLGFSIAGGVGNQHIPGDNSIYVTKIIEGGAAHKDGRLQIGDKILAVNSVGLEDVMHEDAVAALKNTYDVVYLKVAKPSNAYLSDSYAPPDITTSYSQHLDNEISHSSYLGTDYPTAMTPTSPRRYSPVAKDLLGEEDIPREPRRIVIHRGSTGLGFNIVGGEDGEGIFISFILAGGPADLSGELRKGDQILSVNGVDLRNASHEQAAIALKNAGQTVTIIAQYKPEEYSRFEAKIHDLREQLMNSSLGSGTASLRSNPKRGFYIRALFDYDKTKDCGFLSQALSFRFGDVLHVIDAGDEEWWQARRVHSDSETDDIGFIPSKRRVERREWSRLKAKDWGSSSGSQGREDSVLSYETVTQMEVHYARPIIILGPTKDRANDDLLSEFPDKFGSCVPHTTRPKREYEIDGRDYHFVSSREKMEKDIQAHKFIEAGQYNSHLYGTSVQSVREVAEQGKHCILDVSANAVRRLQAAHLHPIAIFIRPRSLENVLEINKRITEEQARKAFDRATKLEQEFTECFSAIVEGDSFEEIYHKVKRVIEDLSGPYIWVPARERL

100-110 kDa

Greater than 80% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCl, 100 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.5.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.