1. 重组蛋白
  2. Cytokines and Growth Factors
  3. Peptide Hormone & Neuropeptides
  4. PTH 蛋白, Human (HEK293, His)

PTH 蛋白, Human (HEK293, His)

目录号: HY-P71060
COA 产品使用指南

PTH 蛋白或甲状旁腺激素通过溶解骨盐和抑制肾脏排泄来提高钙水平。它还刺激成骨细胞中的 [1-14C]-2-脱氧-D-葡萄糖转运和糖原合成,与 PTH1R 受体相互作用并与其 N 末端胞外结构域结合以产生这些作用。PTH 蛋白, Human (HEK293, His) 是重组的 PTH 蛋白,由 HEK293 表达,带有 N-8*His 标签。PTH 蛋白, Human (HEK293, His) 全长 84 个氨基酸,分子量约为 ~15 kDa。

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  • 生物活性

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描述

PTH Protein, or parathyroid hormone, raises calcium levels by dissolving bone salts and inhibiting kidney excretion. It also stimulates [1-14C]-2-deoxy-D-glucose transport and glycogen synthesis in osteoblastic cells, interacting with the PTH1R receptor and binding to its N-terminal extracellular domain for these effects. PTH Protein, Human (HEK293, His) is the recombinant human-derived PTH protein, expressed by HEK293 , with N-8*His labeled tag. The total length of PTH Protein, Human (HEK293, His) is 84 a.a., with molecular weight of ~15 kDa.

研究背景

PTH(甲状旁腺激素)通过促进骨盐溶解并抑制肾脏排泄来提高钙水平。此外,它还能刺激 [1-14C]-2-脱氧-D-葡萄糖 (2DG) 转运并促进成骨细胞中的糖原合成。PTH 通过与 PTH1R 受体相互作用,特别是与受体的 N 末端胞外结构域结合来实现这些效果。

生物活性

Measured by its ability to alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells.  The ED50 this effect is 7.273 ng/mL, corresponding to a specific activity is 1.375×105 units/mg.

  • Measured by its ability to alkaline phosphatase production by MC3T3-E1 mouse preosteoblast cells. The ED50 for this effect is 8.814 ng/mL, corresponding to a specific activity is 1.135×105 units/mg.
种属

Human

表达系统

HEK293

标签

N-8*His

蛋白编号

P01270 (S32-Q115)

基因 ID
蛋白结构
N-term
8*His
PTH (S32-Q115)
Accession # P01270
C-term
同用名
Parathyroid hormone; PTH; Parathormone; Parathyrin; PTH
氨基酸序列

SVSEIQLMHNLGKHLNSMERVEWLRKKLQDVHNFVALGAPLAPRDAGSQRPRKKEDNVLVESHEKSLGEADKADVNVLTKAKSQ

分子量

Approximately 15 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US;may vary elsewhere.

文件资料

PTH Protein, Human (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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目录号:
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