PVR/CD155 蛋白, Human (Biotinylated, HEK293, His-Avi)

The PVR/CD155 protein plays a key role in initiating natural killer (NK) cell effector functions by binding to CD96 and CD226 receptors. These interactions form mature immune synapses that trigger adhesion, lytic granule secretion, interferon-γ (IFN-γ), and cytotoxicity of activated NK cells. PVR/CD155 Protein, Human (Biotinylated, HEK293, His-Avi) is the recombinant human-derived PVR/CD155 protein, expressed by HEK293 , with C-Avi, C-His labeled tag. The total length of PVR/CD155 Protein, Human (Biotinylated, HEK293, His-Avi) is 323 a.a., with molecular weight of 55-70 kDa.

PVR/CD155 蛋白通过与两种不同的 NK 细胞受体 CD96 和 CD226 结合，在协调自然杀伤 (NK) 细胞粘附和启动 NK 细胞效应功能方面发挥着关键作用。这些受体相互作用汇聚在细胞与细胞接触位点，最终在 NK 细胞和靶细胞之间形成成熟的免疫突触。该事件会触发活化的 NK 细胞的粘附、溶解颗粒的分泌、干扰素-γ (IFN-γ) 以及细胞毒性的激活。PVR/CD155还可以促进NK细胞-靶细胞模块交换和PVR向NK细胞的转移，这对于表达高水平PVR的肿瘤细胞尤其重要。在这种情况下，转移机制可能会诱导自相残杀的 NK 细胞激活，为肿瘤提供逃避免疫反应的机制。此外，PVR/CD155 还参与介导肿瘤细胞侵袭和迁移。在微生物感染的情况下，该蛋白质充当脊髓灰质炎病毒的受体，并可能在病毒的轴突运输中发挥作用。该功能涉及通过与 DYNLT1 相互作用将含有病毒颗粒 PVR 的内吞囊泡靶向微管网络，从而促进病毒的轴突逆行转运。

Measured by its binding ability in a functional ELISA. When immobilized Biotinylated Human CD155 His at 1 μg/mL (100μL/Well) on the streptavidin precoated plate (5 μg/mL), can bind Human TIGIT hFc Tag and the EC₅₀ is <10 ng/mL.

Human

HEK293

C-Avi;C-His

P15151 (W21-N343)

5817  [NCBI]

55-70 kDa

Greater than 95% as determined by Tris-Bis PAGE.

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.