RAC1 蛋白, Human (sf9, GST)

The RAC1 protein is a plasma membrane-associated small GTPase that dynamically regulates multiple cellular responses by cycling between an active GTP-bound state and an inactive GDP-bound state. In its active form, RAC1 regulates processes such as secretory function, phagocytosis, cell polarization, neuronal activity, and growth factor-induced membrane ruffle formation. RAC1 Protein, Human (sf9, GST) is the recombinant human-derived RAC1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

RAC1 蛋白是一种质膜相关的小 GTP 酶，在其活性 GTP 结合状态和非活性 GDP 结合状态之间动态循环，对一系列细胞反应进行调节控制。在活性状态下，RAC1 与各种效应蛋白结合，调节分泌功能、凋亡细胞的吞噬作用、上皮细胞极化、神经元粘附、迁移、分化和生长因子诱导的膜皱褶形成等过程。它与细胞质中的 Rho GDI 形成活性异二聚体，称为 sigma 1，参与刺激巨噬细胞中的 NADPH 氧化酶活性。RAC1 对于 SPATA13 介导的细胞迁移以及粘附组装和分解的调节至关重要，它还可以刺激 PKN2 激酶活性。它与 RAB7A 合作调节破骨细胞中褶皱边界的形成，并促进足细胞中 NR3C2 的核穿梭，以实现正常的肾功能。在神经元中，RAC1 参与树突棘形成、突触可塑性、树突棘形态发生和突触形成，凸显了其在神经生物学中的多方面作用。此外，RAC1 通过 PAK1 激活和 F-肌动蛋白稳定，在调节 GABA(A) 受体突触稳定性和 GABA 能抑制性突触传递中发挥着至关重要的作用。RAC1 的亚型 B 在其不依赖于 GEF 的 GDP/GTP 交换和受损的 GTP 水解方面表现出独特的特征，并由 GTP 酶激活蛋白部分恢复，这表明其效应器相互作用中存在微妙的调节机制。

Measured by its ability to catalyze 4 μM substrate GTP that incubate at room temperature for 60 minutes. The specific activity is 15.0 nmol/min/mg.

Human

Sf9 insect cells

N-GST

P63000-1 (M1-C189)

5879  [NCBI]

Full Length of Isoform 1 Mature Protein

Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE

溶液.

Supplied as a 0.22 μm filtered solution of 50 mM Tris, 100 mM NaCl, 1 mM GSH, 10% glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.