1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily T Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands RANKL/CD254
  5. RANKL
  6. RANK L/TNFSF11 蛋白, Human (HEK293, Fc)

RANK L/TNFSF11 蛋白, Human (HEK293, Fc)

目录号: HY-P73682
COA 产品使用指南

RANKL (TNFSF11),一种 II 型跨膜蛋白,是 NF-κB (RANK) 受体的配体。RANKL 是 NF-κB 的激活剂。当与 NF-κB 结合后,其诱导单核细胞/巨噬细胞谱系细胞分化为破骨细胞,并进一步导致破骨细胞前体成熟。因此,RANKL 对于破骨细胞成熟、骨骼建模和骨骼重塑,以及淋巴结 (LN) 的发育至关重要。RANKL/TNFSF11 蛋白, Human (HEK293, Fc) 是一种由 HEK293 产生的重组人RANKL (G63-D244),N 端带 mFc 标签。

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规格 价格 是否有货 数量
5 μg ¥1150 In-stock
20 μg ¥3300 In-stock
100 μg ¥9200 In-stock
250 μg   询价  

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描述

RANKL (TNFSF11), a type II transmembrane protein, is a receptor ligand for NF-κB (RANK). RANKL is an activator of NF-κB. When binding to NF-κB, it induces the differentiation of monocyte/macrophage-lineage cells into osteoclasts and leads to osteoclast precursor maturation. RANKL is critical for osteoclasts maturation, bone modeling, and bone remodeling, as well as the development of lymph nodes (LNs). RANKL/TNFSF11 Protein, Human (HEK293, Fc) is a recombinant human RANKL (G63-D244) with N-terminal mFc tag, which is produced in HEK293 cell[1][2].

研究背景

RANKL (TNFSF11) belongs to TNF family. RANKL is a type II transmembrane protein and is a receptor ligand for NF-κB (RANK). RANKL is an activator of NF-κB. RANKL binds to NF-κB and induces the differentiation of monocyte/macrophage-lineage cells into osteoclasts and leads to osteoclast precursor maturation. In bone tissue, RANKL is expressed by osteoblasts, osteocytes and immune cells, especially in osteoblasts and osteocytes[1]. RANKL is also expressed by T cells and increases proliferation and survival of dendritic cells[2].
Human RANKL shares 82.02% and 84.44% common aa identity with mouse and rat respectively. Human RANKL consists of cytoplasmic domain (1-47), helical domain (48-68), and extracellular domain (69-317). The soluble chain (140-317) is released when cleaved by enzymes such as matrix metalloproteinases (MMP3 or 7) and ADAM[1][3].
RANKL is critical for osteoclasts maturation, bone modeling, and bone remodeling, as well as the development of lymph nodes (LNs)[1].

体外研究
(In Vitro)

RANKL (human, 0-50 ng/mL, 24 h) stimulates migration of a clear cell RCC cell line, Caki-1[4].
RANKL (human, 24 h) stimulates PAa cell migration and invasion[5].

体内研究
(In Vivo)

RANKL (human, 0.4 or 2 mg/kg/day, s.c.) induces high bone turnover and decreases bone volume, density, and strength in C57BL/6J female mice[6].

生物活性

Immobilized mouse mFc-TNFSF11 at 10 μg/mL (100 μL/well) can bind human TNFRSF11B-His and the EC50 is 6.0-14.1 ng/mL. The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells and the ED50 is 16 ng/mL.

种属

Human

表达系统

HEK293

标签

N-mFc

蛋白编号

O14788-2 (G63-D244)

基因 ID
中文名
重组人 RANKL/TNFSF11 蛋白 (HEK293, Fc)
同用名
Tumor necrosis factor ligand superfamily member 11; ODF; RANKL; CD254; TNFSF11
分子量

50-60 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

Lyophilized powder.

组分

Lyophilized a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
RANK L/TNFSF11 Protein, Human (HEK293, Fc)
目录号:
HY-P73682
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