1. 重组蛋白
  2. Cytokines and Growth Factors CD Antigens
  3. TNF Superfamily T Cell CD Proteins Macrophage CD Proteins
  4. TNF Superfamily Ligands RANKL/CD254
  5. RANKL
  6. RANK L/TNFSF11 蛋白, Human (HEK293, mFc)

RANK L/TNFSF11 蛋白, Human (HEK293, mFc)

目录号: HY-P73682
COA 产品使用指南

RANKL (TNFSF11),一种 II 型跨膜蛋白,是 NF-κB 受体激活剂 (RANK) 的配体。RANKL 是 RANK 的激活剂。当与 RANK 结合后,其诱导单核细胞/巨噬细胞谱系细胞分化为破骨细胞,并进一步导致破骨细胞前体成熟。因此,RANKL 对于破骨细胞成熟、骨骼建模和骨骼重塑,以及淋巴结 (LN) 的发育至关重要。RANKL/TNFSF11 蛋白, Human (HEK293, Fc) 是一种由 HEK293 产生的重组人RANKL (G63-D244),N 端带 mFc 标签。

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20 μg ¥2250 In-stock
100 μg ¥6200 In-stock
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描述

RANKL (TNFSF11), a type II transmembrane protein, is a receptor activator of NF-κB (RANK) ligand. RANKL is an activator of RANK. When binding to RANK, it induces the differentiation of monocyte/macrophage-lineage cells into osteoclasts and leads to osteoclast precursor maturation. RANKL is critical for osteoclasts maturation, bone modeling, and bone remodeling, as well as the development of lymph nodes (LNs). RANKL/TNFSF11 Protein, Human (HEK293, Fc) is a recombinant human RANKL (G63-D244) with N-terminal mFc tag, which is produced in HEK293 cell[1][2].

研究背景

RANKL (TNFSF11) 属于 TNF 家族。RANKL 是一种 II 型跨膜蛋白,是 NF-κB (RANK) 配体的受体激活剂。RANKL 是 RANK 的激活剂。RANKL 与 RANK 结合,诱导单核细胞/巨噬细胞谱系细胞分化为破骨细胞,并导致破骨细胞前体成熟。在骨组织中,RANKL 由成骨细胞、骨细胞和免疫细胞表达,尤其是在成骨细胞和骨细胞中表达[1]RANKL 也由 T 细胞表达,可促进树突状细胞的增殖和存活[2]
人类 RANKL 与小鼠和大鼠分别具有 82.02% 和 84.44% 的共同氨基酸同一性。人RANKL由胞质结构域(1-47)、螺旋结构域(48-68)和胞外结构域(69-317)组成。当基质金属蛋白酶(MMP3 或 7)和 ADAM[1][3] 等酶裂解时,可溶性链 (140-317) 被释放。
RANKL 对于破骨细胞成熟、骨建模和骨重塑以及淋巴结 (LN) 的发育至关重要[1]

体外研究
(In Vitro)

RANKL (human, -5 ng/mL, 24 h) stimulates migration of a clear cell RCC cell line, Caki-1[4].
RANKL (human, 24 h) stimulates PAa cell migration and invasion[5].

体内研究
(In Vivo)

RANKL (human, .4 or 2 mg/kg/day, s.c.) induces high bone turnover and decreases bone volume, density, and strength in C57BL/6J female mice[6].

生物活性

1.The bioactivity of hRANKL was determined by measuring the ability of hRANKL to induce TRAP activity in Raw 264.7 cells and the ED50 is 16 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Human TNFRSF11B His at 2 μg/mL (100 μl/well) can bind Human RANKL mFc, the EC50 of Human RANKL mFc is 15-80 ng/mL.

种属

Human

表达系统

HEK293

标签

N-mFc

蛋白编号

O14788-2 (G63-D244)

基因 ID
蛋白结构
N-term
mFc
RANKL (G63-D244)
Accession # O14788-2
C-term
中文名
重组人 RANKL/TNFSF11 蛋白 (HEK293, Fc)
同用名
Tumor necrosis factor ligand superfamily member 11; ODF; RANKL; CD254; TNFSF11
分子量

50-60 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
RANK L/TNFSF11 Protein, Human (HEK293, mFc)
目录号:
HY-P73682
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