RANKL/TNFSF11 蛋白, Human (HEK293, His)

RANKL/TNFSF11 Protein, Human (HEK293, His) is the recombinant human-derived RANKL/TNFSF11 protein, expressed by HEK293, with N-His tag.

RANKL/TNFSF11 是一种细胞因子，可与 TNFRSF11B/OPG 和 TNFRSF11A/RANK 结合。它是破骨细胞分化和激活因子，能增强树突状细胞刺激初始 T 细胞增殖的能力，可能是 T 细胞与树突状细胞相互作用的重要调节因子，并在 T 细胞依赖性免疫应答调控中发挥作用。此外，它在恶性肿瘤体液性高钙血症中可能参与增强骨吸收。通过激活破骨前体细胞中的多种信号通路（尤其是诱导细胞内 Ca ²⁺ 浓度的持久振荡，进而激活 NFATC1 并转位至细胞核，诱导破骨细胞特异性基因转录以促进破骨细胞分化），RANKL/TNFSF11 诱导破骨细胞生成。在破骨细胞分化过程中，它还以 TMEM64 和 ATP2A2 依赖性方式激活 CREB1 并生成线粒体 ROS，这对正常破骨细胞生成至关重要（By similarity）。

Human

HEK293

N-His

AAC51762.1 (G64-D245)

/

Partial

Approximately 28-35 kDa,based on SDS-PAGE under reducing conditions,due to the glycosylation.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.