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  3. RBP4 蛋白, Canine (HEK293, His)

RBP4 蛋白, Canine (HEK293, His)

目录号: HY-P76001
COA 产品使用指南

RBP4 蛋白是脂质运载蛋白家族成员之一,是循环中疏水分子视黄醇 (又称维生素 a) 的主要转运蛋白。RBP4 蛋白, Canine (HEK293, His) 是重组的 RBP4 蛋白,由 HEK293 表达,带有 C-His 标签。RBP4 蛋白, Canine (HEK293, His) 全长 183 个氨基酸,分子量约为 ~23 kDa。

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  • 生物活性

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描述

RBP4 Protein is a member of the lipocalin family and the major transport protein of the hydrophobic molecule retinol, also known as vitamin A, in the circulation. RBP4 Protein, Canine (HEK293, His) is the recombinant canine-derived RBP4 protein, expressed by HEK293 , with C-His labeled tag. The total length of RBP4 Protein, Canine (HEK293, His) is 183 a.a., with molecular weight of ~23 kDa.

研究背景

RBP4 蛋白是脂质运载蛋白家族成员之一,是循环中疏水分子视黄醇 (又称维生素 a) 的主要转运蛋白。RBP4 使视黄醇结合和视黄醇跨膜转运活性成为可能。RBP4 参与视黄醇转运并位于细胞外间隙[1][2][3]

生物活性

Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. The 50% binding concentration (ED50) is 2.771 μM, as measured under the described conditions.

种属

Canine

表达系统

HEK293

标签

C-His

蛋白编号

XP_038295882 (E19-L201)

基因 ID

477775  [NCBI]

蛋白结构
N-term
RBP4 (E19-L201)
Accession # XP_038295882
His
C-term
同用名
Plasma retinol-binding protein; PRBP; RBP
氨基酸序列

ESDCRVSNFQVKKNFDKARFAGTWYAMAKKDPEGLFLQDNIVAEFSVDENGRMSATAKGRVRLLNNWDVCADMVGTFTDTEDPAKFKMKYWGVASFLQKGNDDHWIIDTDYDTYAVQYSCRLLNLDGTCADSYSFVFSRDPNGLPLEAQKIVRQRQEELCLARQYRLIVHNGYCDGRSEPNTL

分子量

Approximately 23 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

RBP4 Protein, Canine (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
RBP4 Protein, Canine (HEK293, His)
目录号:
HY-P76001
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