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  3. RBP4 蛋白, Rat (HEK293, His)

RBP4 蛋白, Rat (HEK293, His)

目录号: HY-P74598
COA 产品使用指南

RBP4 蛋白转运血浆中的视黄醇,将其从肝脏输送到周围组织。它与全反式视黄醇结合,将其转移至 STRA6 进行细胞膜运输。RBP4 与 TTR 相互作用,防止肾脏过滤,并进一步帮助 STRA6 进行视黄醇转运。RBP4 蛋白, Rat (HEK293, His) 是重组的 RBP4 蛋白,由 HEK293 表达,带有 C-His 标签。RBP4 蛋白, Rat (HEK293, His) 全长 183 个氨基酸,分子量约为 ~24 kDa。

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描述

RBP4 Protein transports retinol in blood plasma, delivering it from the liver to peripheral tissues. It binds to all-trans retinol, transferring it to STRA6 for cell membrane transport. RBP4 interacts with TTR, preventing kidney filtration, and further aids STRA6 in retinol transport. RBP4 Protein, Rat (HEK293, His) is the recombinant rat-derived RBP4 protein, expressed by HEK293 , with C-His labeled tag. The total length of RBP4 Protein, Rat (HEK293, His) is 183 a.a., with molecular weight of ~24 kDa.

研究背景

RBP4 蛋白是一种视黄醇结合蛋白,参与血浆中视黄醇的运输。它在将视黄醇从肝脏储存输送到外周组织方面发挥着至关重要的作用。RBP4 与全反式视黄醇结合并将其转移至 STRA6,从而促进视黄醇跨细胞膜的转运。RBP4 还与 TTR 相互作用,阻止其通过肾小球过滤。此外,它与 STRA6 相互作用,进一步促进其在视黄醇运输中的功能。

生物活性

Measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. The 50% binding concentration (BC50) is 0.293 μM, as measured under the described conditions.

种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

P04916 (E19-L201)

基因 ID

25703  [NCBI]

蛋白结构
N-term
RBP4 (E19-L201)
Accession # P04916
His
C-term
同用名
Plasma retinol-binding protein; PRBP; RBP
氨基酸序列

ERDCRVSSFRVKENFDKARFSGLWYAIAKKDPEGLFLQDNIIAEFSVDEKGHMSATAKGRVRLLSNWEVCADMVGTFTDTEDPAKFKMKYWGVASFLQRGNDDHWIIDTDYDTFALQYSCRLQNLDGTCADSYSFVFSRDPNGLTPETRRLVRQRQEELCLERQYRWIEHNGYCQSRPSRNSL

分子量

Approximately 24 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

RBP4 Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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