REG-3 alpha/REG3A 蛋白, Mouse (HEK293, His)

REG-3 α/REG3A proteins exhibit identical protein-binding, oligosaccharide-binding, and peptidoglycan-binding activities. It negatively regulates keratinocyte differentiation and positively regulates keratinocyte proliferation and wound healing, emphasizing its role in skin-related processes. REG-3 alpha/REG3A Protein, Mouse (HEK293, His) is the recombinant mouse-derived REG-3 alpha/REG3A protein, expressed by HEK293 , with C-His labeled tag. The total length of REG-3 alpha/REG3A Protein, Mouse (HEK293, His) is 149 a.a., with molecular weight of ~18 kDa.

REG-3 α (REG3A) 蛋白预计表现出多种功能，包括相同的蛋白结合活性、寡糖结合活性和肽聚糖结合活性。它参与角质形成细胞分化的负向调节、角质形成细胞增殖的正向调节以及伤口愈合的正向调节，凸显了其在皮肤相关过程中的作用。REG3A 预计位于细胞外区域并在细胞外空间活跃，在肠道和胰腺中显着表达。人类中直系同源基因的存在，例如 REG3G（再生家族成员 3 gamma），强调了其功能的进化保守性。值得注意的是，大肠和十二指肠等组织中的偏向表达进一步强调了其在不同生理环境中的特定作用。

Measured in a cell proliferation assay using H4 human neuroglioma cells. The ED₅₀ for this effect is 0.202 μg/mL, corresponding to a specific activity is 4.960×10³ Unit/mg.

Mouse

HEK293

C-His

NP_035389 (E27-Q175)

19694  [NCBI]

EDFQKEVPSPRTSCPMGYKAYRSHCYALVMTPKSWFQADLVCQKRPSGHLVSILSGGEASFVSSLVNGRVDNYQDIWIGLHDPTMGQQPNGGGWEWSNSDVLNYLNWDGDPSSTVNRGHCGSLTASSGFLKWGDYYCDGTLPFVCKFKQ

Approximately 18 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.