RGMA 蛋白, Human (HEK293, His)

The RGMA protein is a member of the repulsion guidance molecule (RGM) family and plays multiple roles in nervous system development and function. It controls neural tube closure, inhibits neurite growth, inhibits neuronal branching, and regulates the formation of mature synapses. RGMA Protein, Human (HEK293, His) is the recombinant human-derived RGMA protein, expressed by HEK293 , with C-His labeled tag. The total length of RGMA Protein, Human (HEK293, His) is 422 a.a., with molecular weight of ~36 kDa.

RGMA 蛋白是排斥引导分子 (RGM) 家族的成员，在发育中和成人神经系统中发挥着多种作用。它控制头部神经管闭合，抑制神经突生长，抑制皮质神经元分支，并调节成熟突触的形成。RGMA 与其受体 NEO1/neogenin 结合后，通过 UNC5B-ARHGEF12/LARG-PTK2/FAK1 级联触发 RHOA-ROCK1/Rho 激酶信号通路的激活，导致神经元生长锥塌陷并抑制神经突生长出来的。此外，RGMA 与 NEO1/neogenin 的相互作用影响 HRAS-PTK2/FAK1-AKT1 通路，导致 HRAS 失活。RGMA 作为骨形态发生蛋白 (BMP) 辅助受体，可能通过 SMAD1、SMAD5 和 SMAD8 发出信号。其复杂的相互作用网络，包括与 NEO1、BMP2 和 BMP4 的相互作用网络，强调了 RGMA 在协调神经系统发育和功能所必需的关键分子事件方面的重要性。

Human

HEK293

C-His

Q96B86 (M1-G422)

56963  [NCBI]

Approximately 36 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.