2. 重组蛋白
  3. Enzymes & Regulators
  4. Hydrolases (EC 3)
  5. Ribonuclease J/RnJ 蛋白, Synechocystis sp. (His-SUMO)

Ribonuclease J/RnJ 蛋白, Synechocystis sp. (His-SUMO)

目录号: HY-P71470
产品使用指南

核糖核酸酶 J/RnJ 蛋白是一种具有 5'-3' 核酸外切酶活性和潜在核酸内切酶功能的酶，对于核糖体 RNA (rRNA) 的成熟至关重要。它还在 mRNA 成熟/衰变中发挥作用，有助于复杂的 RNA 加工，确保细胞环境中的精确成熟和质量控制。Ribonuclease J/RnJ 蛋白, Synechocystis sp. (His-SUMO) 是重组的 核糖核酸酶 J/RnJ 蛋白，由 E. coli 表达，带有 N-His, N-SUMO 标签。Ribonuclease J/RnJ 蛋白, Synechocystis sp. (His-SUMO) 全长 640 个氨基酸，分子量约为 ~86.4 kDa。

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描述

Ribonuclease J/RnJ Proteinas, an enzyme with 5'-3' exonuclease activity and potential endonuclease function, is crucial in ribosomal RNA (rRNA) maturation. It also plays a role in mRNA maturation/decay, contributing to intricate RNA processing, ensuring precise maturation, and quality control in the cellular environment. Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) is the recombinant Ribonuclease J/RnJ protein, expressed by E. coli , with N-His, N-SUMO labeled tag. The total length of Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) is 640 a.a., with molecular weight of ~86.4 kDa.
研究背景

核糖核酸酶 J (RnJ) 是一种具有 5'-3' 核酸外切酶活性的酶，具有作为核酸内切酶的潜在附加功能。它在核糖体 RNA (rRNA) 的成熟过程中发挥着至关重要的作用，并与某些生物体中 mRNA 的成熟和/或衰变有关。这种多功能 RNase 是复杂的 RNA 加工过程中不可或缺的一部分，有助于细胞环境中的精确成熟和质量控制机制。
种属

Others
表达系统

E. coli
标签

N-His;N-SUMO
蛋白编号

P54123 (1M-640S)
基因 ID

23611654  [NCBI]
蛋白结构
N-term
6*His-SUMO
RnJ (1M-640S)
Accession # P54123
C-term
同用名
rnj; slr0551; Ribonuclease J; RNase J
氨基酸序列

MAKNTQTQALKILPLGGLHEIGKNTCVFEYDDEILLLDAGLAFPTDDMHGVNVVLPDMTYLRENREKIKGMVVTHGHEDHIGGIAYHLKQFDIPIIYGPRLAMALLRDKLEEAGMLERTNLQTVSPREMVRLGKSFVVEFIRNTHSIADSYCLAIHTPLGVVMHSGDFKIDHTPIDGEFFDLQKVAEYGEKGVLCLLSDSTNAEVPGITPSEASVIPNLDRVFSQAEGRLMVTTFASSVHRVNIILSLAQKHQRKVAVVGRSMLNVIAHARKLGYIKCPDNLFVPLKAARNLPDQQQLILTTGSQGEPLAAMTRISNGEHPQIKIRQGDTVVFSANPIPGNTIAVVNTIDRLMMQGANVIYGKHQGIHVSGHASQEEHKMLLALTRPKFFVPVHGEHRMLVKHSQMAQAQGIPSENIVIVNNGDVIELTGDRIRVAGQVPSGIELVDQAGIVHESTMAERQQMAEDGLVTVAAALSKTGTLLAYPEVHCRGVVMTIQPKLLEELIVRTIENFLTERWSEFTHGSNGSTEVSWNALQKELESSLQRLIKRELQSSPMVLLMLQTDTPIELDQVPQNVSTVSATSATPAPRKKVVLTKTPEPKVKAKPEKKVVTTAEPSAQPVSTTKVYRRSRKRSTTSVSS

分子量

Approximately 86.4 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE.
内毒素含量

<1 EU/μg, determined by LAL method.
文件资料
产品使用指南 (1213 KB)

Ribonuclease J/RnJ Protein, Synechocystis sp. (His-SUMO) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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