RIPK1 蛋白, Mouse (C-His)

RIPK1 Protein, Mouse (C-His) is the recombinant mouse-derived RIPK1 protein, expressed by E. coli, with C-6*His tag.

p-RIPK1 是一种丝氨酸-苏氨酸激酶，是 TNF 介导的凋亡 (apoptosis)、坏死性凋亡 (necroptosis) 和炎症通路的关键调控因子。它具有激酶活性依赖和非依赖的双重功能：激酶活性参与调控细胞死亡 (如通过形成复合体 IIa 和 IIb 分别诱导凋亡和坏死性凋亡)，而其支架功能则通过结合 TNF-R1 信号复合物 (TNF-RSC/complex I) 促进细胞存活并激活 NF-kappa-B 通路。正常情况下，p-RIPK1 能抑制 RIPK3 依赖的坏死性凋亡 (通过 FADD 招募 CASP8 切割 RIPK1) 和胚胎发育中的异常凋亡 (通过阻止 TRADD-FADD 相互作用)。此外，它还参与炎症反应，促进 IL6 等促炎细胞因子的转录。类似功能包括：与 RIPK3 发生相互自磷酸化和转磷酸化；在 TNF-α 依赖模式下磷酸化 DAB2IP 以激活 MAP3K5-JNK 凋亡级联。

Mouse

E. coli

C-6*His

Q60855 (M1-S656)

19766

Full Length

MQPDMSLDNIKMASSDLLEKTDLDSGGFGKVSLCYHRSHGFVILKKVYTGPNRAEYNEVLLEEGKMMHRLRHSRVVKLLGIIIEEGNYSLVMEYMEKGNLMHVLKTQIDVPLSLKGRIIVEAIEGMCYLHDKGVIHKDLKPENILVDRDFHIKIADLGVASFKTWSKLTKEKDNKQKEVSSTTKKNNGGTLYYMAPEHLNDINAKPTEKSDVYSFGIVLWAIFAKKEPYENVICTEQFVICIKSGNRPNVEEILEYCPREIISLMERCWQAIPEDRPTFLGIEEEFRPFYLSHFEEYVEEDVASLKKEYPDQSPVLQRMFSLQHDCVPLPPSRSNSEQPGSLHSSQGLQMGPVEESWFSSSPEYPQDENDRSVQAKLQEEASYHAFGIFAEKQTKPQPRQNEAYNREEERKRRVSHDPFAQQRARENIKSAGARGHSDPSTTSRGIAVQQLSWPATQTVWNNGLYNQHGFGTTGTGVWYPPNLSQMYSTYKTPVPETNIPGSTPTMPYFSGPVADDLIKYTIFNSSGIQIGNHNYMDVGLNSQPPNNTCKEESTSRHQAIFDNTTSLTDEHLNPIRENLGRQWKNCARKLGFTESQIDEIDHDYERDGLKEKVYQMLQKWLMREGTKGATVGKLAQALHQCCRIDLLNHLIRASQS

Approximately 80-100 kDa, based on SDS-PAGE under reducing conditions.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.