S100A5 蛋白, Mouse (His)

S100A5 Protein, with versatile binding properties, interacts with calcium, zinc, and copper. Each subunit can bind two calcium ions or two copper ions along with one zinc ion. Calcium and copper ions compete for the same binding sites, revealing dynamic molecular interactions in S100A5. Structurally, the protein forms a homodimer, suggesting its dimeric form mediates functional activities in cellular processes. S100A5 Protein, Mouse (His) is the recombinant mouse-derived S100A5 protein, expressed by E. coli , with N-His labeled tag. The total length of S100A5 Protein, Mouse (His) is 93 a.a., with molecular weight of ~13 kDa.

S100A5 蛋白表现出多种结合特性，可与钙、锌和铜相互作用。每个亚基能够同时结合两个钙离子或两个铜离子以及一个锌离子。有趣的是，钙离子和铜离子竞争相同的结合位点，表明 S100A5 分子相互作用中存在动态相互作用。在结构上，该蛋白质以同二聚体形式存在，其二聚体形式可能在介导其在细胞过程中的功能活动中发挥作用。

Measured in a cell proliferation assay using T24 cells. The ED₅₀ for this effect is 13.14 ng/mL, corresponding to a specific activity is 7.61×10⁴ units/mg.

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  Measured in a cell proliferation assay using T24 cells. The ED₅₀ for this effect is 13.14 ng/mL, corresponding to a specific activity is 7.61×10⁴ units/mg.

Mouse

E. coli

N-10*His

P63084 (M1-K93)

20199  [NCBI]

METPLEKALTTMVTTFHKYSGREGSKLTLSRKELKELIKTELSLAEKMKESSIDNLMKSLDKNSDQEIDFKEYSVFLTTLCMAYNDFFLEDNK

Approximately 13 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.