1. 重组蛋白
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Plpro
  5. SARS-CoV-2 Plpro/Papain-like protease 蛋白 (His)

SARS-CoV-2 Plpro/Papain-like protease 蛋白 (His)

目录号: HY-P76021
产品使用指南

SARS-CoV-2 NSP3 (NSP3) 是一种非结构蛋白,可与病毒 RNA、核衣壳蛋白及其他病毒蛋白结合,参与多蛋白加工。NSP3 在先天免疫应答拮抗中也有重要作用,并负责从 pp1a 和 pp1ab 的 N 端释放NSP1、NSP2 和 NSP3。SARS-CoV-2 Plpro/Papain-like protease 蛋白 (His) 是重组的 SARS-CoV-2 Plpro/木瓜蛋白酶样蛋白酶蛋白,由 E. coli 表达,带有 N-His 标签。SARS-CoV-2 Plpro/Papain-like protease 蛋白 (His) 全长 317 个氨基酸,分子量约为 ~36.79 kDa。

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描述

SARS-CoV-2 NSP3 (NSP3) is one of non-structure protein that binds to viral RNA, nucleocapsid protein, as well as other viral proteins, and contributes in polyprotein processing. NSP3 has also an important role in innate immune response antagonism and is responsible for release of NSP1, NSP2, and NSP3 from the N-terminal region of pp1a and pp1ab. SARS-CoV-2 Plpro/Papain-like protease Protein (His) is the recombinant Virus-derived SARS-CoV-2 Plpro/Papain-like protease protein, expressed by E. coli , with N-His labeled tag. The total length of SARS-CoV-2 Plpro/Papain-like protease Protein (His) is 317 a.a., with molecular weight of ~36.79 kDa.

研究背景

SARS-CoV-2 NSP3 (NSP3) 是一种非结构蛋白,可与病毒 RNA、核衣壳蛋白及其他病毒蛋白结合,参与多蛋白加工。NSP3 在先天免疫应答拮抗中也有重要作用,并负责从 pp1a 和 pp1ab 的 N 端释放NSP1、NSP2 和 NSP3。NSP3 在刺激病毒 mRNA 的翻译中起着重要作用,这些 mRNA 被加帽修饰但没有聚腺苷化,而是在 NSP3 识别的 3' 处保守序列 'GACC' 终止,NSP3 与宿主 PABPC1 竞争 EIF4G1 结合。NSP3 与宿主 EIF4G1 的相互作用稳定了 EIF4E-EIF4G1 的相互作用,从而促进了加帽 mRNA 翻译的启动[1][2]

种属

Virus

表达系统

E. coli

标签

N-His

蛋白编号

YP_009725299 (E1564-V1880)

基因 ID

43740578  [NCBI]

蛋白结构
N-term
His
SARS-CoV-2 Plpro (E1564-V1880)
Accession # YP_009725299
C-term
同用名
SARS-CoV-2 (2019-nCoV) Plpro/papain-like protease-His Protein
分子量

Approximately 36.79 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SARS-CoV-2 Plpro/Papain-like protease Protein (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SARS-CoV-2 Plpro/Papain-like protease Protein (His)
目录号:
HY-P76021
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