SARS-CoV-2 S 蛋白 RBD (194a.a, HEK293, C-His)

SARS-CoV-2 S1 Protein, in its Spike protein S1 form, initiates infection by attaching the virion to the cell membrane through host receptor interaction. Simultaneously, Spike protein S2' acts as a viral fusion peptide, revealing itself after S2 cleavage during virus endocytosis. SARS-CoV-2 S Protein RBD (194a.a, HEK293, C-His) is the recombinant Virus-derived SARS-CoV-2 S protein RBD, expressed by HEK293 , with C-6*His labeled tag. The total length of SARS-CoV-2 S Protein RBD (194a.a, HEK293, C-His) is 194 a.a., with molecular weight of ~31.9 kDa.

SARS-CoV-2 S1 蛋白在病毒感染的早期阶段发挥着至关重要的作用。刺突蛋白 S1 通过与宿主受体相互作用，促进病毒粒子附着在细胞膜上，从而启动感染过程。这种初始结合事件对于病毒随后进入宿主细胞至关重要。同时，刺突蛋白S2'作为病毒融合肽，在病毒内吞过程中S2裂解后发挥作用。S2'的暴露是病毒融合过程中的关键步骤，使病毒膜与内体膜融合，并促进病毒遗传物质释放到宿主细胞的细胞质中。这些 S1 和 S2' 功能的协同作用强调了 SARS-CoV-2 S1 蛋白在介导病毒进入和融合（病毒生命周期中的关键步骤）中的重要性。

Measured by its binding ability in a functional ELISA. Immobilized Mouse SARS-CoV-2 S1, at 2 μg/mL (100 μL/well) can bind Biotinylated ACE2 protein. The ED₅₀ for this effect is 40.46 ng/mL, corresponding to a specific activity is 2.47×10^5 Unit/mg.

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  Measured by its binding ability in a functional ELISA. Immobilized Mouse SARS-CoV-2 S1, at 2 μg/mL (100 μL/well) can bind Biotinylated ACE2 protein. The ED₅₀ for this effect is 40.46 ng/mL, corresponding to a specific activity is 2.47×10⁵ Unit/mg.

Virus

HEK293

C-6*His

QHD43416.1 (N331-V524)

43740568  [NCBI]

NITNLCPFGEVFNATRFASVYAWNRKRISNCVADYSVLYNSASFSTFKCYGVSPTKLNDLCFTNVYADSFVIRGDEVRQIAPGQTGKIADYNYKLPDDFTGCVIAWNSNNLDSKVGGNYNYLYRLFRKSNLKPFERDISTEIYQAGSTPCNGVEGFNCYFPLQSYGFQPTNGVGYQPYRVVVLSFELLHAPATVHHHHHH

Approximately 31.9 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.