1. 重组蛋白
  2. Viral Proteins
  3. SARS-CoV-2 Proteins
  4. SARS-CoV-2 Spike Proteins
  5. SARS-CoV-2 S Protein RBD
  6. SARS-CoV-2 S 蛋白 RBD (HEK293, Fc)

SARS-CoV-2 S 蛋白 RBD (HEK293, Fc)

目录号: HY-P73397
产品使用指南

SARS-Cov-2 S glyco蛋白是一个 SARS-Cov-2 S 糖蛋白。SARS-Cov-2 S glycoprotein 的氨基酸序列 1261-1267a.a 是跨膜形式。SARS-Cov-2 S glyco蛋白是高度糖基化的三聚体,其每个原聚体由1260个氨基酸 (残基14-1273) 组成,分为四个结构域: n 端结构域 (NTD),c 端结构域 (CTD,也称为受体结合结构域,RBD) 和两个子结构域 (SD1 和 SD2)。SARS-CoV-2 S 蛋白 RBD (HEK293, Fc) 是重组的 SARS-CoV-2 S 蛋白 RBD,由 HEK293 表达,带有 C-hFc 标签。SARS-CoV-2 S 蛋白 RBD (HEK293, Fc) 全长 223 个氨基酸,分子量约为 ~51.8 kDa。

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描述

The SARS-Cov-2 S glycoprotein is a SARS-Cov-2 S glycoprotein. The amino acid sequence 1261-1267a.a of the SARS-Cov-2 S glycoprotein is transmembrane. The SARS-Cov-2 S glycoprotein is a highly glycosylated trimer, each of which consists of 1260 amino acids (residues 14-1273), divided into four structural domains: the n-terminal domain (NTD), the c-terminal domain (CTD, also known as the receptor-binding domain, RBD), and two subdomains (SD1 and SD2). SARS-CoV-2 S Protein RBD (HEK293, Fc) is the recombinant Virus-derived SARS-CoV-2 S protein RBD, expressed by HEK293 , with C-hFc labeled tag. The total length of SARS-CoV-2 S Protein RBD (HEK293, Fc) is 223 a.a., with molecular weight of ~51.8 kDa.

研究背景

SARS-Cov-2 是一种包膜阳性单链RNA病毒,可导致2019冠状病毒病(COVID-19)。
SARS-CoV-2 具有 4 种结构蛋白,包括包膜蛋白 (E)、刺突或表面糖蛋白 (S)、膜蛋白(M) 和核衣壳蛋白 (N)。
SARS-Cov-2 S glycoprotein 位于病毒颗粒的外部,使冠状病毒具有冠状外观。
ARS-Cov-2 S glycoprotein 可以介导病毒颗粒附着并进入宿主细胞,是疫苗开发、抗体治疗和基于抗原的诊断测试的重要靶标[1][2][3][4][5]

种属

Virus

表达系统

HEK293

标签

C-hFc

蛋白编号

YP_009724390.1 (R319-F541)

基因 ID

43740568  [NCBI]

蛋白结构
N-term
Spike RBD (R319-F541)
Accession # YP_009724390.1
hFc
C-term
同用名
2019-nCov RBD Protein; 2019-nCoV Spike RBD Protein; S protein RBD; 2019-nCoV S protein RBD
分子量

Approximately 51.8 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SARS-CoV-2 S Protein RBD (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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