1. 重组蛋白
  2. Cytokines and Growth Factors
  3. SCF 蛋白, Human (sf9, His)

SCF 蛋白, Human (sf9, His)

目录号: HY-P73410
产品使用指南

SCF 蛋白是 KIT 受体型蛋白酪氨酸激酶的配体,调节多种细胞过程,包括生存、增殖、造血、干细胞维持、配子发生、肥大细胞发育、迁移和黑素生成。与 KIT 结合可激活涉及 PIK3R1、AKT1、GRB2、RAS、RAF1、MAP 激酶、STAT 家族成员和 PLCG1 的信号通路,产生二酰基甘油和肌醇 1,4,5-三磷酸。SCF 蛋白, Human (sf9, His) 是重组的 SCF 蛋白,由 Sf9 insect cells 表达,带有 C-His 标签。SCF 蛋白, Human (sf9, His) 全长 164 个氨基酸,分子量约为 ~22 kDa。

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描述

SCF proteins are ligands for the KIT receptor-type protein tyrosine kinase and regulate a variety of cellular processes, including survival, proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration, and melanogenesis. Binding to KIT activates signaling pathways involving PIK3R1, AKT1, GRB2, RAS, RAF1, MAP kinase, STAT family members, and PLCG1, producing diacylglycerol and inositol 1,4,5-trisphosphate. SCF Protein, Human (sf9, His) is the recombinant human-derived SCF protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of SCF Protein, Human (sf9, His) is 164 a.a., with molecular weight of ~22 kDa.

研究背景

GMP 干细胞因子 (SCF) 蛋白作为受体型蛋白酪氨酸激酶 KIT 的配体,在多种细胞过程的调节中发挥着关键作用。其功能涵盖细胞存活和增殖、造血、干细胞维持、配子发生、肥大细胞发育、迁移和黑素生成的控制。与 KIT 结合后,GMP SCF 激活多种信号通路,包括 PIK3R1 的磷酸化和随后激酶 AKT1 的激活。这种相互作用还会触发涉及 GRB2、RAS、RAF1 和 MAP 激酶 MAPK1/ERK2 和/或 MAPK3/ERK1 的信号级联。此外,GMP SCF 和 KIT 促进 STAT 家族成员(STAT1、STAT3 和 STAT5)以及 PLCG1 的激活,从而产生细胞信号分子二酰甘油和肌醇 1,4,5-三磷酸。GMP SCF 与其他细胞因子(可能是白细胞介素)协同作用,与 KIT 形成非共价连接的同二聚体和异四聚体,促进 KIT 二聚化并随后通过自磷酸化激活。

种属

Human

表达系统

Sf9 insect cells

标签

C-His

蛋白编号

P21583 (E26-A189)

基因 ID
蛋白结构
N-term
SCF (E26-A189)
Accession # P21583
His
C-term
同用名
SCF; Hematopoietic growth factor KL; MGF; Mast Cell Growth Factor
氨基酸序列

EGICRNRVTNNVKDVTKLVANLPKDYMITLKYVPGMDVLPSHCWISEMVVQLSDSLTDLLDKFSNISEGLSNYSIIDKLVNIVDDLVECVKENSSKDLKKSFKSPEPRLFTPEEFFRIFNRSIDAFKDFVVASETSDCVVSSTLSPEKDSRVSVTKPFMLPPVA

分子量

Approximately 22 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SCF Protein, Human (sf9, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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