SCF 蛋白, Mouse (P.pastoris)

SCF Protein, Mouse (P.pastoris) is the ligand for the receptor-type protein-tyrosine kinase KIT. It plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis.

胚胎着床是一个复杂的过程，需要胚胎和子宫内膜的相互作用。几种生长因子和细胞因子似乎参与了这一过程。干细胞因子（SCF）及其受体c-kit调节生殖细胞的增殖和存活，在卵泡发育中发挥重要作用。在胚胎发育过程中，SCF和c-kit对于生殖细胞的存活和增殖以及向性腺的迁移至关重要。c-kit mRNA 在原始生殖细胞中表达，而 SCF 转录物则沿着其向生殖嵴的迁移途径表达^[1] 。

The ED₅₀ is <10.0 ng/mL as measured by human TF-1 cells, corresponding to a specific activity of >1.0× 10⁵ units/mg.

Mouse

P. pastoris

Tag Free

P20826 (K26-A189)

17311  [NCBI]

MKEICGNPVTDNVKDITKLVANLPNDYMITLNYVAGMDVLPSHCWLRDMVIQLSLSLTTLLDKFSNISEGLSNYSIIDKLGKIVDDLVLCMEENAPKNIKESPKRPETRSFTPEEFFSIFNRSIDAFKDFMVASDTSDCVLSSTLGPEKDSRVSVTKPFMLPPVA

Approximately 18.4 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized after extensive dialysis against 50 mM Tris, pH 8.0.

<0.2 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Mitsunari M, et al. The potential role of stem cell factor and its receptor c-kit in the mouse blastocyst implantation. Mol Hum Reprod. 1999 Sep;5(9):874-9.  [Content Brief]