Semaphorin-4A/SEMA4A 蛋白, Human (HEK293, His)

Semaphorin-4A/SEMA4A is the cell surface receptor for PLXNB1, PLXNB2, PLXNB3, and PLXND1 and is essential for intercellular signaling. It induces TIMD2 phosphorylation, inhibits angiogenesis, and collapses axonal growth cones. Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is the recombinant human-derived Semaphorin-4A/SEMA4A protein, expressed by HEK293 , with C-His labeled tag. The total length of Semaphorin-4A/SEMA4A Protein, Human (HEK293, His) is 683 a.a., with molecular weight of 75-80 kDa.

Semaphorin-4A (SEMA4A) 是 PLXNB1、PLXNB2、PLXNB3 和 PLXND1 的细胞表面受体，在细胞间信号传导中发挥着关键作用。这种多面蛋白参与谷氨酸能和 GABA 能突触发育的调节，以 PLXNB1 依赖性方式培育抑制性突触，并以 PLXNB2 依赖性方式培育兴奋性突触。此外，SEMA4A 通过启动抗原特异性 T 细胞、促进 Th1 T 辅助细胞的分化以及促进 TIMD2 的磷酸化来促进适应性免疫。此外，SEMA4A 对血管生成发挥抑制作用，诱导轴突生长锥塌陷，并通过提供指定轴突生长无法到达的区域的局部信号来阻碍轴突延伸。与 PLXNB1、PLXNB2、PLXNB3、PLXND1 和 TIMD2 的相互作用强调了其复杂的作用，强调了其参与多种细胞过程。

Human

HEK293

C-His

Q9H3S1 (M1-H683)

64218  [NCBI]

MALPALGLDPWSLLGLFLFQLLQLLLPTTTAGGGGQGPMPRVRYYAGDERRALSFFHQKGLQDFDTLLLSGDGNTLYVGAREAILALDIQDPGVPRLKNMIPWPASDRKKSECAFKKKSNETQCFNFIRVLVSYNVTHLYTCGTFAFSPACTFIELQDSYLLPISEDKVMEGKGQSPFDPAHKHTAVLVDGMLYSGTMNNFLGSEPILMRTLGSQPVLKTDNFLRWLHHDASFVAAIPSTQVVYFFFEETASEFDFFERLHTSRVARVCKNDVGGEKLLQKKWTTFLKAQLLCTQPGQLPFNVIRHAVLLPADSPTAPHIYAVFTSQWQVGGTRSSAVCAFSLLDIERVFKGKYKELNKETSRWTTYRGPETNPRPGSCSVGPSSDKALTFMKDHFLMDEQVVGTPLLVKSGVEYTRLAVETAQGLDGHSHLVMYLGTTTGSLHKAVVSGDSSAHLVEEIQLFPDPEPVRNLQLAPTQGAVFVGFSGGVWRVPRANCSVYESCVDCVLARDPHCAWDPESRTCCLLSAPNLNSWKQDMERGNPEWACASGPMSRSLRPQSRPQIIKEVLAVPNSILELPCPHLSALASYYWSHGPAAVPEASSTVYNGSLLLIVQDGVGGLYQCWATENGFSYPVISYWVDSQDQTLALDPELAGIPREHVKVPLTRVSGGAALAAQQSYWPH

75-80 kDa

Greater than 90% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.