SENP1 Catalytic Domain 蛋白, Human (His)

SENP1 Catalytic Domain Protein hydrolyzes SUMO propeptides (SUMO1, SUMO2, and SUMO3), maturing them. It also deconjugates SUMO1, SUMO2, and SUMO3 from target proteins, impacting their activities. Interacts with MTA1 and CCAR2, influencing SIRT1 interaction and transcriptional activities, showcasing its regulatory role in SUMOylation processes. SENP1 Catalytic Domain Protein, Human (His) is the recombinant human-derived SENP1 Catalytic Domain protein, expressed by E. coli , with N-His labeled tag. The total length of SENP1 Catalytic Domain Protein, Human (His) is 230 a.a., with molecular weight of ~30 kDa.

SENP1 催化域蛋白是一种蛋白酶，通过催化两个基本功能在 SUMO 途径中发挥关键作用。首先，它水解小泛素样修饰剂 (SUMO) 前肽（包括 SUMO1、SUMO2 和 SUMO3）C 末端的 α 连接肽键，从而形成成熟蛋白质。其次，它通过裂解成熟 SUMO 的 C 端甘氨酸和目标蛋白的赖氨酸 ε-氨基之间的 ε 连接肽键，从目标蛋白中解离 SUMO1、SUMO2 和 SUMO3。SENP1 催化域蛋白已知可将 SUMO1 与 HIPK2、HDAC1 和 BHLHE40/DEC1 解偶联，从而导致其转录抑制活性降低。它还使 SUMO1 与 CLOCK 解偶联，降低其转录激活活性。此外，SENP1 催化结构域蛋白可将 SUMO2 与 MTA1 以及 SUMO1 与 METTL3 解偶联。它还使 CCAR2 去苏酰化，从而减少其与 SIRT1 的相互作用。SENP1 催化域蛋白通过各自的 N 末端与 MTA1 和 CCAR2 相互作用。

Human His6-SENP1 Catalytic Domain is a SUMO-specific deconjugating enzyme with an initial Human His6-SENP1 Catalytic Domain concentration of 50-500 nM. A 15 minute pre-incubation with 10 mM DTT is recommended to achieve maximum activity.

Human

E. coli

N-6*His

Q9P0U3-1 (D415-L644)

29843  [NCBI]

DSEDEFPEITEEMEKEIKNVFRNGNQDEVLSEAFRLTITRKDIQTLNHLNWLNDEIINFYMNMLMERSKEKGLPSVHAFNTFFFTKLKTAGYQAVKRWTKKVDVFSVDILLVPIHLGVHWCLAVVDFRKKNITYYDSMGGINNEACRILLQYLKQESIDKKRKEFDTNGWQLFSKKSQEIPQQMNGSDCGMFACKYADCITKDRPINFTQQHMPYFRKRMVWEILHRKLL

Approximately 30 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of in 50 mM HEPES, 200 mM NaCl, pH 7.4, 20% Glycerol.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.