Serpin F1 蛋白, Mouse (HEK293, His)

Serpin F1 is a neurotrophin that significantly induces extensive neuronal differentiation of retinoblastoma cells and exhibits potent anti-angiogenic effects. Its unique conformation lacks the S (stress) to R (relaxation) transition typical of active serpins, resulting in a lack of serpin activity. Serpin F1 Protein, Mouse (HEK293, His) is the recombinant mouse-derived Serpin F1 protein, expressed by HEK293 , with C-His labeled tag. The total length of Serpin F1 Protein, Mouse (HEK293, His) is 398 a.a., with molecular weight of 60-65 kDa.

Serpin F1 是一种神经营养蛋白，是视网膜母细胞瘤细胞广泛神经元分化的关键诱导物。值得注意的是，它对血管生成具有有效的抑制作用。以其独特的构象特征而著称，Serpin F1 不会经历活性丝氨酸蛋白酶抑制剂典型的从 S（应激）到 R（松弛）的转变，从而导致缺乏丝氨酸蛋白酶抑制活性。此外，它与 PNPLA2 的相互作用可刺激 PNPLA2 的磷脂酶 A2 活性，强调 Serpin F1 在细胞过程中的多方面作用。

Measured by its binding ability in a functional ELISA. Immobilized mouse SERPINF1-His at 10 μg/mL (100 μL/well) can bind human GST-CSNK2A1 with a linear range of 0.31-2.5 μg/mL.

Mouse

HEK293

C-His

P97298 (Q20-T417)

20317  [NCBI]

MQALVLLLWTGALLGHGSSQNVPSSSEGSPVPDSTGEPVEEEDPFFKVPVNKLAAAVSNFGYDLYRLRSSASPTGNVLLSPLSVATALSALSLGAEHRTESVIHRALYYDLITNPDIHSTYKELLASVTAPEKNLKSASRIVFERKLRVKSSFVAPLEKSYGTRPRILTGNPRVDLQEINNWVQAQMKGKIARSTREMPSALSILLLGVAYFKGQWVTKFDSRKTTLQDFHLDEDRTVRVPMMSDPKAILRYGLDSDLNCKIAQLPLTGSMSIIFFLPLTVTQNLTMIEESLTSEFIHDIDRELKTIQAVLTVPKLKLSFEGELTKSLQDMKLQSLFESPDFSKITGKPVKLTQVEHRAAFEWNEEGAGSSPSPGLQPVRLTFPLDYHLNQPFLFVLRDTDTGALLFIGRILDPSST

60-65 kDa

Greater than 95% as determined by reducing SDS-PAGE

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.