Serum Albumin 蛋白, Rat (HEK293, His)

Serum Albumin Protein, Rat (HEK293, His) is the recombinant rat-derived Serum Albumin protein, expressed by HEK293, with N-His tag.

Serum Albumin 是一种多功能血浆蛋白，主要功能是调节血液的胶体渗透压（colloidal osmotic pressure）。它能结合水、Ca²⁺、Na(+)、K(+)、脂肪酸（fatty acids）、激素（hormones）、胆红素（bilirubin）和药物（drugs）。作为血浆中主要的锌转运蛋白（zinc transporter），通常结合约 80% 的血浆锌（By similarity）。同时，它也是血浆中钙和镁的主要转运蛋白（calcium and magnesium transporter），结合约 45% 的循环钙和镁（By similarity）。其 Asp-273 位点是锌和钙的共同结合位点，表明血液中锌和钙的转运存在交互作用（By similarity）。亲和力顺序为锌 > 钙 > 镁（By similarity）。此外，它能结合细菌铁载体肠杆菌素（enterobactin），抑制大肠杆菌（E.coli）从转铁蛋白（transferrin）摄取铁，从而限制肠道细菌如大肠杆菌对铁的利用和生长（By similarity），但不影响细菌铁载体 aerobactin 介导的铁摄取（By similarity）。

Rat

HEK293

N-8*His

P02770 (E25-A608)

Full Length of Mature Protein

Predicted band size: 67.52 kDa; Observed band size: 65-70 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.