1. 重组蛋白
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  3. SHH 蛋白, Mouse (C25II)

SHH 蛋白, Mouse (C25II)是一种小鼠重组 sonic hedgehog 的变体。

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2 μg ¥320 In-stock
5 μg ¥600 In-stock
10 μg ¥960 In-stock
50 μg ¥2500 In-stock
100 μg ¥4250 In-stock
500 μg ¥12000 询价
1 mg ¥17000 询价
> 1 mg   询价  

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  • 生物活性

  • 技术参数

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  • 产品资料

  • 参考文献

描述

SHH Protein, Mouse (C25II) is a recombinant mouse sonic hedgehog variant.

研究背景

Sonic Hedgehog (SHH) 是一种分泌因子。该蛋白质通过切割和翻译后修饰进行了大量修饰,并且其从生产细胞中的释放也可以通过与 Dispatched、Scube2 和硫酸乙酰肝素蛋白聚糖的相互作用来调节。Sonic hidehog (SHH) 是小鼠[1][2] 中刺猬蛋白 (Hh) 通路的主要配体。

生物活性

The ED50 is <2 μg/mL as measured by C3H/10T1/2 (CCL-226) cells, corresponding to a specific activity of >500 units/mg.

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

Q62226 (C25-G198, C25II)

基因 ID
蛋白结构
N-term
SHH (C25-G198, C25II)
Accession # Q62226
C-term
蛋白长度

Partial

中文名
SHH 蛋白
同用名
rMuSonic Hedgehog, C25II; Hhg1
氨基酸序列

IIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG

分子量

Approximately 19.7-23 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

SHH Protein, Mouse (C25II) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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SHH Protein, Mouse (C25II)
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HY-P7409
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