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  3. SHH 蛋白, Mouse (C25II)

SHH 蛋白, Mouse (C25II)

目录号: HY-P7409
COA 产品使用指南

SHH 蛋白, Mouse (C25II)是一种小鼠重组 sonic hedgehog 的变体。

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规格 价格 是否有货 数量
10 μg ¥1600 In-stock
50 μg ¥3800 In-stock
100 μg   询价  
250 μg   询价  

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描述

SHH Protein, Mouse (C25II) is a recombinant mouse sonic hedgehog variant.

研究背景

Sonic Hedgehog (SHH) is a secreted factor. The protein is heavily modified through cleavage and posttranslational modification and its release from producing cells can also be modulated by interactions with Dispatched, Scube2, and heparan sulfate proteoglycan. Sonic hedgehog (SHH) is the main ligand of hedgehog (Hh) pathway in mouse[1][2].

生物活性

The ED50 is <2 μg/mL as measured by C3H/10T1/2 (CCL-226) cells, corresponding to a specific activity of >500 units/mg.

种属

Mouse

表达系统

E. coli

标签

Tag Free

蛋白编号

Q62226 (G26-G198)

基因 ID

20423  [NCBI]

中文名
重组小鼠 Sonic Hedgehog, C25II
同用名
rMuSonic Hedgehog, C25II; Hhg1
氨基酸序列

IIGPGRGFGKRRHPKKLTPLAYKQFIPNVAEKTLGASGRYEGKITRNSERFKELTPNYNPDIIFKDEENTGADRLMTQRCKDKLNALAISVMNQWPGVKLRVTEGWDEDGHHSEESLHYEGRAVDITTSDRDRSKYGMLARLAVEAGFDWVYYESKAHIHCSVKAENSVAAKSGG

分子量

Approximately 19.7 kDa

性状

Lyophilized powder.

组分

Lyophilized after extensive dialysis against PBS.

内毒素含量

<0.2 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
参考文献

SHH Protein, Mouse (C25II) 相关分类

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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