Shiga toxin 2B 蛋白, E. coli (His)

Shiga toxin 2B Proteinas, specifically its B subunit, binds the holotoxin to target cell receptors, such as globotriaosylceramide (Gb3) on human intestinal microvilli. Its capability to recognize and engage with these receptors underscores its pivotal role in initiating the holotoxin's interaction with the target cell surface, setting the stage for subsequent cellular responses and internalization processes. Shiga toxin 2B Protein, E. coli (His) is the recombinant E. coli-derived Shiga toxin 2B protein, expressed by E. coli , with N-His labeled tag. The total length of Shiga toxin 2B Protein, E. coli (His) is 89 a.a., with molecular weight of ~16 kDa.

志贺毒素 2B 蛋白，特别是其 B 亚基，在将全毒素与靶细胞表面的特定受体结合方面发挥着关键作用。值得注意的是，这种结合相互作用针对受体，例如三酰基神经酰胺 (Gb3)，特别是位于人肠道微绒毛上的受体。B 亚基识别并与这些特定受体结合的能力强调了其在促进全毒素与靶细胞表面相互作用的初始阶段的关键功能，为随后的细胞反应和内化过程奠定了基础。

E.coli

E. coli

N-His

Q93EY4 (M1-D89)

/

Approximately 16 kDa

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.