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SHP-2 蛋白, Mouse (HEK293, His)

目录号: HY-P74549
COA 产品使用指南

SHP-2 蛋白在多种受体和细胞质酪氨酸激酶的下游发挥作用,参与从细胞表面到细胞核的信号转导。它积极调节 MAPK 通路并使 GAB1、ARHGAP35、EGFR、ROCK2、CDC73 和 SOX9 等关键蛋白去磷酸化。此外,SHP-2 使 NEDD9/CAS-L 去磷酸化以调节细胞过程。SHP-2 蛋白, Mouse (HEK293, His) 是重组的 SHP-2 蛋白,由 HEK293 表达,带有 C-His 标签。SHP-2 蛋白, Mouse (HEK293, His) 全长 593 个氨基酸,分子量约为 ~65 kDa。

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5 μg ¥430 In-stock
10 μg ¥740 In-stock
50 μg ¥2060 In-stock
100 μg ¥3500 In-stock
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描述

SHP-2 Protein functions downstream of diverse receptor and cytoplasmic tyrosine kinases, participating in signal transduction from the cell surface to the nucleus. It positively regulates the MAPK pathway and dephosphorylates key proteins like GAB1, ARHGAP35, EGFR, ROCK2, CDC73, and SOX9. Additionally, SHP-2 dephosphorylates NEDD9/CAS-L to modulate cellular processes. SHP-2 Protein, Mouse (HEK293, His) is the recombinant mouse-derived SHP-2 protein, expressed by HEK293 , with C-His labeled tag. The total length of SHP-2 Protein, Mouse (HEK293, His) is 593 a.a., with molecular weight of ~65 kDa.

研究背景

SHP-2 蛋白在各种受体和细胞质蛋白酪氨酸激酶的下游发挥作用,参与从细胞表面到细胞核的信号转导。它正向调节 MAPK 信号转导通路,并对 GAB1、ARHGAP35、EGFR、ROCK2(位于“Tyr-722”,增强其 RhoA 结合活性)、CDC73 和酪氨酸磷酸化 NEDD9/CAS-L 等底物表现出去磷酸化活性。值得注意的是,SHP-2 通过使其酪氨酸残基去磷酸化,在 SOX9 失活中发挥作用,从而促进骨化。通过其多方面的去磷酸化活性,SHP-2 在调节涉及细胞生长、分化和细胞对各种刺激的反应的关键信号传导途径方面发挥着关键作用。

生物活性

Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site. The specific activity is 34.1661 µmol/min/mg, measured under the described conditions.

种属

Mouse

表达系统

HEK293

标签

C-His

蛋白编号

P35235-2 (M1-R593)

基因 ID

19247  [NCBI]

蛋白结构
N-term
SHP-2 (M1-R593)
Accession # P35235-2
His
C-term
同用名
Tyrosine-protein phosphatase non-receptor type 11; SH-PTP2; SHP-2; Ptpn11
氨基酸序列

MTSRRWFHPNITGVEAENLLLTRGVDGSFLARPSKSNPGDFTLSVRRNGAVTHIKIQNTGDYYDLYGGEKFATLAELVQYYMEHHGQLKEKNGDVIELKYPLNCADPTSERWFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHYKKNPMVETLGTVLQLKQPLNTTRINAAEIESRVRELSKLAETTDKVKQGFWEEFETLQQQECKLLYSRKEGQRQENKNKNRYKNILPFDHTRVVLHDGDPNEPVSDYINANIIMPEFETKCNNSKPKKSYIATQGCLQNTVNDFWRMVFQENSRVIVMTTKEVERGKSKCVKYWPDEYALKEYGVMRVRNVKESAAHDYTLRELKLSKVGQGNTERTVWQYHFRTWPDHGVPSDPGGVLDFLEEVHHKQESIVDAGPVVVHCSAGIGRTGTFIVIDILIDIIREKGVDCDIDVPKTIQMVRSQRSGMVQTEAQYRFIYMAVQHYIETLQRRIEEEQKSKRKGHEYTNIKYSLVDQTSGDQSPLPPCTPTPPCAEMREDSARVYENVGLMQQQRSFR

分子量

Approximately 65 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

SHP-2 Protein, Mouse (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SHP-2 Protein, Mouse (HEK293, His)
目录号:
HY-P74549
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