1. 重组蛋白
  2. Receptor Proteins
  3. Siglec
  4. Siglec-10
  5. Siglec-10 蛋白, Human (HEK293, Fc)

Siglec-10 蛋白, Human (HEK293, Fc)

目录号: HY-P72476
COA 产品使用指南

Siglec-10 蛋白被认为是一种假定的粘附分子,可介导唾液酸依赖性细胞结合,优先选择 α-2,3- 或 α-2,6- 连接的唾液酸。其唾液酸识别位点可能被顺式相互作用掩盖。Siglec-10 蛋白, Human (HEK293, Fc) 是重组的 Siglec-10 蛋白,由 HEK293 表达,带有 C-hFc 标签。Siglec-10 蛋白, Human (HEK293, Fc) 全长 530 个氨基酸,分子量为 110-130 kDa。

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5 μg ¥650 In-stock
10 μg ¥1100 In-stock
50 μg ¥3000 In-stock
100 μg   询价  

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描述

Siglec-10 protein is thought to be a putative adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked by cis interactions. Siglec-10 Protein, Human (HEK293, Fc) is the recombinant human-derived Siglec-10 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-10 Protein, Human (HEK293, Fc) is 530 a.a., with molecular weight of 110-130 kDa.

研究背景

Siglec-10 蛋白被认为是一种推定的粘附分子,在唾液酸依赖性细胞结合中发挥作用,显示出对 α-2,3- 或 α-2,6- 连接唾液酸的偏好。Siglec-10 的唾液酸识别位点可能由于与同一细胞表面上的唾液酸的顺式相互作用而被掩蔽。在免疫反应中,它似乎充当抑制性受体,诱导配体诱导的酪氨酸磷酸化并通过其 SH2 结构域招募细胞质磷酸酶,通过信号分子的去磷酸化来阻断信号转导。Siglec-10 依赖于 PTPN6/SHP-1,参与 B 细胞抗原受体信号传导的负向调节。与 CD24 结合,它可能参与选择性抑制对危险相关分子模式 (DAMP)(例如 HMGB1、HSP70 和 HSP90)的免疫反应。Siglec-10 与 CD24 协同调节自然杀伤 (NK) 细胞的免疫反应,并在控制自身免疫方面发挥作用。在 CD8-α(+) 树突状细胞启动适应性免疫反应期间,Siglec-10 通过损害 MHC I 类肽复合物的形成来抑制交叉呈递,从而招募 PTPN6/SHP-1 并促进吞噬体酸化。Siglec-10 与多种蛋白质相互作用,包括 PTPN6/SHP-1、NCF1、CD24、HMGB1、RIGI、CBL 和 PTPN11。

生物活性

1.Immobilized Human Siglec-10 at 2 μg/mL (100 μL/well) can bind Anti-Siglec-10 Antibody, The ED50 is 8.695 ng/mL, corresponding to a specific activity is 1.15×105 units/mg.
2.Immobilized Anti-Human Siglec10 mAb at 2 μg/mL (100 μl/well) can bind Human Siglec-10-Fc. The ED50 of Human Siglec-10-Fc is 44.9 ng/mL.

  • Immobilized Human Siglec-10 at 2 μg/mL (100 μL/well) can bind Anti-Siglec-10  Antibody,  The ED50 for this effect 8.695 ng/mL,corresponding to a specific activity is 1.15×105units/mg.
种属

Human

表达系统

HEK293

标签

C-hFc

蛋白编号

Q96LC7 (M17-T546)

基因 ID
蛋白结构
N-term
Siglec-10 (M17-T546)
Accession # Q96LC7
hFc
C-term
同用名
Sialic acid-binding Ig-like lectin 10; Siglec-10; SIGLEC10; SLG2
氨基酸序列

MDGRFWIRVQESVMVPEGLCISVPCSFSYPRQDWTGSTPAYGYWFKAVTETTKGAPVATNHQSREVEMSTRGRFQLTGDPAKGNCSLVIRDAQMQDESQYFFRVERGSYVRYNFMNDGFFLKVTALTQKPDVYIPETLEPGQPVTVICVFNWAFEECPPPSFSWTGAALSSQGTKPTTSHFSVLSFTPRPQDHNTDLTCHVDFSRKGVSAQRTVRLRVAYAPRDLVISISRDNTPALEPQPQGNVPYLEAQKGQFLRLLCAADSQPPATLSWVLQNRVLSSSHPWGPRPLGLELPGVKAGDSGRYTCRAENRLGSQQRALDLSVQYPPENLRVMVSQANRTVLENLGNGTSLPVLEGQSLCLVCVTHSSPPARLSWTQRGQVLSPSQPSDPGVLELPRVQVEHEGEFTCHARHPLGSQHVSLSLSVHYSPKLLGPSCSWEAEGLHCSCSSQASPAPSLRWWLGEELLEGNSSQDSFEVTPSSAGPWANSSLSLHGGLSSGLRLRCEAWNVHGAQSGSILQLPDKKGLIST

分子量

110-130 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM Tris-HCl, 6% Sucrose, 4% Mannitol, 50mM NaCl, 0.05% Tween 80, pH 8.0.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

Siglec-10 Protein, Human (HEK293, Fc) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Siglec-10 Protein, Human (HEK293, Fc)
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HY-P72476
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