Siglec-10 蛋白, Mouse (HEK293, Fc)

Siglec-10 protein is an adhesion molecule that mediates sialic acid-dependent cell binding, preferentially selecting α-2,3- or α-2,6-linked sialic acid. Its sialic acid recognition site may be masked through cis interactions. Siglec-10 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived Siglec-10 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of Siglec-10 Protein, Mouse (HEK293, Fc) is 525 a.a., with molecular weight of 110-135 kDa.

Siglec-10 蛋白被认为是一种推定的粘附分子，作为唾液酸依赖性细胞结合的介体，优先结合 α-2,3- 或 α-2,6- 连接的唾液酸。唾液酸识别位点可能通过与同一细胞表面上的唾液酸的顺式相互作用而被掩蔽。在免疫反应中，Siglec-10 作为抑制性受体发挥作用，实现配体诱导的酪氨酸磷酸化，并通过其 SH2 结构域招募细胞质磷酸酶，通过信号分子的去磷酸化来阻断信号转导。值得注意的是，它参与 B 细胞抗原受体信号传导的负调节，特别作用于 B1 细胞，抑制 Ca(2+) 信号传导、细胞扩张和抗体分泌。这种抑制依赖于 PTPN6/SHP-1。Siglec-10 与 CD24 结合，可能参与选择性抑制对危险相关分子模式 (DAMP) 的免疫反应，并调节自然杀伤 (NK) 细胞的免疫反应。此外，它在控制自身免疫方面发挥着作用，并且在 CD8-α(+) 树突状细胞启动适应性免疫反应期间，通过损害 MHC I 类肽复合物的形成来抑制交叉呈递。此外，在 RNA 病毒感染微生物的情况下，Siglec-10 通过 PTPN11/SHP-2 促进 CBL 依赖性泛素化和降解，从而抑制巨噬细胞中的 RIG-I 信号传导。Siglec-10 的多方面作用凸显了它在多种细胞过程和免疫调节中的复杂参与。

Mouse

HEK293

C-hFc

Q80ZE3 (M19-K543)

243958  [NCBI]

MESYFLQVQRIVKAQEGLCIFVPCSFSSPEGKWLNRSPLYGYWFKGIRKPSLSFPVATNNKDKVLEWEARGRFQLLGDISKKNCSLLIKDVQWGDSTNYFFRMERGFERFSFKEEFRLQVEALTQKPDIFIPEVLEPGEPVTVVCLFSWTFNQCPAPSFSWMGDAVSFQESRPHTSNYSVLSFIPGLQHHDTELTCQLDFSRMSTQRTVRLRVAYAPRSLAISIFHDNVSVPDLHENPSHLEVQQGQSLRLLCTADSQPPATLSWVLEDQVLSWSSPVGSRTLALELPWVKAGDSGHYTCQAENRLGSQQHTLDLSVLYPPQDLRVTVSQANRTVLEILRNAISLPVLEGQSLCLVCVTYSNPPANVSWAWVTQTLIPIQSSEPGVLELPLVQREHEGEFTCAAQNPLGAQRISLSLSVHYPPQMSSPSCSWEAKGLHCNCSSRAWPAPSLRWRLGEGLLEGNSSNASFTVTFSSLGPWVNSSLSLLQELGPSLWLSCESWNTHGAQTTSVLLLPDKDSATAFSK

110-135 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.