1. 重组蛋白
  2. CAR-T related Proteins CD Antigens Receptor Proteins
  3. NK Cell CD Proteins Macrophage CD Proteins Monocyte CD Proteins Stem Cell CD Proteins Dendritic Cell CD Proteins Siglec
  4. CD33 Siglec-3/CD33
  5. Siglec-3/CD33 蛋白, Human (HEK293, Fc-His)

Siglec-3/CD33 蛋白, Human (HEK293, Fc-His)

目录号: HY-P72472
COA 产品使用指南

Siglec-3/CD33 是一种唾液酸结合凝集素,对介导细胞间相互作用和免疫细胞静止至关重要。它优先选择唾液酸而不是特定的粘蛋白,形成同二聚体,在磷酸化后与 PTPN6/SHP-1 和 PTPN11/SHP-2 相互作用。CD33 还通过其 C 末端参与 C1QA,激活 CD33 抑制基序。Siglec-3/CD33 蛋白, Human (HEK293, Fc-His) 是重组的 Siglec-3/CD33 蛋白,由 HEK293 表达,带有 C-hFc, C-6*His 标签。Siglec-3/CD33 蛋白, Human (HEK293, Fc-His) 全长 242 个氨基酸,分子量约为 ~80 kDa。

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规格 价格 是否有货 数量
10 μg ¥1280 In-stock
50 μg ¥3520 In-stock
100 μg   询价  

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描述

Siglec-3/CD33, a sialic-acid-binding lectin, crucially mediates cell-cell interactions and immune cell quiescence. Preferring sialic acid on specific mucins, it forms homodimers, interacting with PTPN6/SHP-1 and PTPN11/SHP-2 upon phosphorylation. CD33 also engages C1QA through its C-terminus, activating CD33 inhibitory motifs. Siglec-3/CD33 Protein, Human (HEK293, Fc-His) is the recombinant human-derived Siglec-3/CD33 protein, expressed by HEK293 , with C-hFc, C-6*His labeled tag. The total length of Siglec-3/CD33 Protein, Human (HEK293, Fc-His) is 242 a.a., with molecular weight of ~80 kDa.

研究背景

Siglec-3/CD33 是一种唾液酸结合免疫球蛋白样凝集素,在介导细胞间相互作用和维持免疫细胞处于静息状态方面发挥着至关重要的作用。它表现出优先将唾液酸结合到特定粘蛋白的短 O 连接聚糖上。该蛋白通过二硫键形成同二聚体,并在磷酸化后与信号分子(如 PTPN6/SHP-1 和 PTPN11/SHP-2)相互作用。此外,CD33 通过其 C 末端与 C1QA 结合,导致 CD33 抑制基序的激活。

种属

Human

表达系统

HEK293

标签

C-hFc;C-6*His

蛋白编号

AAH28152.1 (D18-H259)

基因 ID

945  [NCBI]

蛋白结构
N-term
CD33 (D18-H259)
Accession # AAH28152.1
hFc-6*His
C-term
同用名
Myeloid Cell Surface Antigen CD33; Sialic Acid-Binding Ig-Like Lectin 3; Siglec-3; gp67; CD33; SIGLEC3
氨基酸序列

DPNFWLQVQESVTVQEGLCVLVPCTFFHPIPYYDKNSPVHGYWFREGAIISRDSPVATNKLDQEVQEETQGRFRLLGDPSRNNCSLSIVDARRRDNGSYFFRMERGSTKYSYKSPQLSVHVTDLTHRPKILIPGTLEPGHSKNLTCSVSWACEQGTPPIFSWLSAAPTSLGPRTTHSSVLIITPRPQDHGTNLTCQVKFAGAGVTTERTIQLNVTYVPQNPTTGIFPGDGSGKQETRAGVVH

分子量

Approximately 80 kDa

纯度

Greater than 95% as determined by reducing SDS-PAGE.

性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, 2 mM EDTA, pH 7.2.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
Siglec-3/CD33 Protein, Human (HEK293, Fc-His)
目录号:
HY-P72472
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