1. 重组蛋白
  2. Enzymes & Regulators
  3. Transferases (EC 2)
  4. GST 蛋白, Schistosoma Japonicum

GST 蛋白, Schistosoma Japonicum

目录号: HY-P70270
COA 产品使用指南

GST(谷胱甘肽 S 转移酶)蛋白对于将还原型谷胱甘肽与疏水性亲电子试剂结合至关重要,这对于细胞解毒和防御至关重要。GST 同工酶在寄生虫的解毒系统中发挥着核心作用,表明其具有至关重要的防御作用。此外,GST 可能会增强寄生虫肠道中血红素的溶解度,突出其对细胞和生物过程的多方面贡献。GST 蛋白, Schistosoma Japonicum 是重组的 日本血吸虫 GST 蛋白,由 E. coli 表达,不带标签。GST 蛋白, Schistosoma Japonicum 全长 218 个氨基酸,分子量约为 ~28.0 kDa。

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5 μg ¥350 In-stock
10 μg ¥500 In-stock
50 μg ¥1500 In-stock
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  • 生物活性

  • 技术参数

  • 产品性质

  • 产品资料

描述

The GST (Glutathione S-transferase) protein is vital for conjugating reduced glutathione to hydrophobic electrophiles, essential for cellular detoxification and defense. GST isoenzymes play a central role in parasites' detoxification systems, indicating a crucial defense role. Additionally, GST may enhance haematin solubility in parasite guts, highlighting its multifaceted contributions to cellular and organismal processes. GST Protein, Schistosoma Japonicum is the recombinant GST protein, expressed by E. coli , with tag free. The total length of GST Protein, Schistosoma Japonicum is 218 a.a., with molecular weight of ~28.0 kDa.

研究背景

GST(谷胱甘肽 S 转移酶)蛋白是还原型谷胱甘肽与多种疏水性亲电子试剂结合的细胞过程中不可或缺的一部分,这是解毒和细胞防御的关键机制。除了在一般解毒过程中的作用外,GST 同工酶还是寄生虫解毒系统中的关键成分,表明其在其防御机制中发挥着核心作用。此外,有迹象表明 GST 可能具有其他功能,例如增强寄生虫肠道中血红素的溶解度,强调其对细胞和有机体过程的多方面贡献。

生物活性

<12 units/mg, is defined as the amount of enzyme that conjugate 1.0 u mole of 1-chloro-2,4-dinitrobenzene (CDNB) with reduced glutathione per minute at at 37°C.

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

P08515 (M1-K218)

基因 ID

/

蛋白结构
N-term
GST (M1-K218)
Accession # P08515
C-term
中文名
重组血吸虫GST蛋白
同用名
rSchGlutathione S-transferase class-mu 26 kDa isozyme; Glutathione S-transferase class-mu 26 kDa isozyme; GST 26; Sj26 antigen; SjGST
氨基酸序列

MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK

分子量

Approximately 28.0 kDa

纯度
  • Greater than 95% as determined by reducing SDS-PAGE.
性状

无菌粉末.

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

GST Protein, Schistosoma Japonicum 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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