SLC25A4 蛋白, Human (His)

SLC25A4 Protein, Human (His) is the recombinant human-derived SLC25A4 protein, expressed by E. coli cell-free system, with N-10*His tag.

SLC25A4 是一种 ADP:ATP 逆向转运蛋白，介导 ADP 进入线粒体基质以合成 ATP，并将 ATP 输出到细胞外以供能。它通过交替访问机制在胞质开放状态（c-state）和基质开放状态（m-state）之间循环，间歇性地将单一底物结合位点暴露于线粒体内膜的胞质侧或基质侧（By similarity）。除了 ADP:ATP 逆向转运活性外，它还参与线粒体解偶联和线粒体通透性转换孔（mPTP）活性。作为质子转运体，它通过解偶联电子传递链和 ATP 合酶中的质子流动来降低 ATP 生成效率并引发线粒体产热（By similarity）。其质子转运活性受 ADP:ATP 逆向转运活性抑制，表明 SLC25A4/ANT1 通过维持 ATP 生成（ADP:ATP 逆向转运活性）与产热（质子转运活性）之间的平衡来调控线粒体能量输出（By similarity）。质子转运活性需要游离脂肪酸作为辅因子，但不转运脂肪酸（By similarity）。此外，它在 mPTP 开放中起关键作用，但尚不清楚它是否构成 mPTP 的孔形成组分或仅调控其功能（By similarity）。它还独立于 ADP:ATP 逆向转运活性调控线粒体自噬：通过与 TIMM44 相互作用抑制 TIMM23，从而稳定 PINK1 以促进线粒体自噬（By similarity）。

Human

E. coli Cell-free

N-10*His

P12235 (G2-V298)

291

Full Length of Mature Protein

GDHAWSFLKDFLAGGVAAAVSKTAVAPIERVKLLLQVQHASKQISAEKQYKGIIDCVVRIPKEQGFLSFWRGNLANVIRYFPTQALNFAFKDKYKQLFLGGVDRHKQFWRYFAGNLASGGAAGATSLCFVYPLDFARTRLAADVGKGAAQREFHGLGDCIIKIFKSDGLRGLYQGFNVSVQGIIIYRAAYFGVYDTAKGMLPDPKNVHIFVSWMIAQSVTAVAGLVSYPFDTVRRRMMMQSGRKGADIMYTGTVDCWRKIAKDEGAKAFFKGAWSNVLRGMGGAFVLVLYDEIKKYV

Approximately 30-38 kDa, based on SDS-PAGE under reducing conditions.

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.22 μm filtered solution of PBS, 0.05% Brij78,6% Trehalose, pH7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.