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  3. SMAC/Diablo 蛋白, Human (His)

SMAC/Diablo 蛋白, Human (His)

目录号: HY-P73416
COA 产品使用指南

SMAC/Diablo 蛋白是细胞凋亡的核心参与者,并激活细胞色素 c/Apaf-1/caspase-9 通路中的 caspase。它拮抗凋亡蛋白抑制剂 (IAP)、抑制 BIRC6/bruce 与半胱天冬酶的结合并破坏 XIAP/BIRC4 的稳定性。SMAC/Diablo 蛋白, Human (His) 是重组的 SMAC/Diablo 蛋白,由 E. coli 表达,带有 C-His 标签。SMAC/Diablo 蛋白, Human (His) 全长 184 个氨基酸,分子量约为 ~22 kDa。

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描述

SMAC/Diablo protein is a core participant in apoptosis and activates caspases in the cytochrome c/Apaf-1/caspase-9 pathway. It antagonizes inhibitors of apoptosis proteins (IAPs), inhibits the binding of BIRC6/bruce to caspases and destabilizes XIAP/BIRC4. SMAC/Diablo Protein, Human (His) is the recombinant human-derived SMAC/Diablo protein, expressed by E. coli , with C-His labeled tag. The total length of SMAC/Diablo Protein, Human (His) is 184 a.a., with molecular weight of ~22 kDa.

研究背景

SMAC/Diablo 蛋白通过激活细胞色素 c/Apaf-1/caspase-9 通路中的 caspase,在细胞凋亡中发挥关键作用。其作用方式涉及抵消凋亡蛋白抑制剂(IAP)的抑制作用。SMAC/Diablo 通过阻止 BIRC6/bruce 与半胱天冬酶的结合,有效地阻碍 BIRC6/bruce 的活性。此外,它通过降低 XIAP/BIRC4 的稳定性和细胞凋亡抑制能力来发挥其调节影响。这是通过泛素-蛋白酶体途径促进 XIAP/BIRC4 泛素化和随后的降解来实现的。值得注意的是,SMAC/Diablo 破坏了 XIAP/BIRC4 和经过加工的 caspase-9 之间的相互作用,从而促进 caspase-3 的激活。从本质上讲,SMAC/Diablo 是控制细胞凋亡过程的复杂分子机制中的关键协调者。

生物活性

Measured by its binding ability in a functional ELISA. Immobilized Human SMAC at 10 μg/mL (100 μL/well) can bind Biotinylated XIAP. The ED50 for this effect is 0.328 μg/mL.

种属

Human

表达系统

E. coli

标签

C-His

蛋白编号

Q9NR28 (A56-D239)

基因 ID
蛋白结构
N-term
SMAC (A56-D239)
Accession # Q9NR28
His
C-term
中文名
重组人SMAC蛋白
同用名
SMAC; Diablo homolog, mitochondrial; DIABLO
氨基酸序列

AVPIAQKSEPHSLSSEALMRRAVSLVTDSTSTFLSQTTYALIEAITEYTKAVYTLTSLYRQYTSLLGKMNSEEEDEVWQVIIGARAEMTSKHQEYLKLETTWMTAVGLSEMAAEAAYQTGADQASITARNHIQLVKLQVEEVHQLSRKAETKLAEAQIEELRQKTQEEGEERAESEQEAYLRED

分子量

Approximately 22 kDa

纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

SMAC/Diablo Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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