SMAD2 蛋白, Human (His-SUMO)

SMAD2 protein, an R-SMAD, acts as a transcriptional modulator activated by TGF-beta and activin type 1 receptor kinases. It binds TRE elements, regulating TGF-beta target genes. Forming a complex with SMAD4, it promotes transcription, positively regulating PDPK1 and showing potential tumor suppressor functions in colorectal carcinoma. SMAD2 interacts with various proteins, participating in signal transduction and transcriptional regulation. SMAD2 Protein, Human (His-SUMO) is the recombinant human-derived SMAD2 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of SMAD2 Protein, Human (His-SUMO) is 466 a.a., with molecular weight of ~68.2 kDa.

SMAD2 蛋白作为受体调节的 SMAD (R-SMAD) 起作用，充当由 TGF-β（转化生长因子）和激活素 1 型受体激酶激活的细胞内信号转导器和转录调节剂。它与 TGF-β 调节的众多基因的启动子区域中的 TRE 元件结合，并在形成 SMAD2/SMAD4 复合物后激活转录。SMAD2 在牙乳头细胞中促进 TGFB1 介导的成牙本质细胞分化基因的转录中发挥作用。此外，它还通过刺激 PDPK1 激酶与 14-3-3 蛋白 YWHAQ 的解离来正向调节 PDPK1 激酶活性，充当负调节剂。有证据表明其在结直肠癌中具有作为肿瘤抑制因子的潜在功能。SMAD2 在没有 TGF-β 存在的情况下以单体形式存在，并在细胞核中与 co-SMAD、SMAD4 形成异二聚体，构成反式激活复合物 SMAD2/SMAD4。它与多种蛋白质相互作用，包括 ZFYVE9、TAZ/WWRT1、FOXH1、SNW1、CREB 结合蛋白 (CBP)、EP300、SNON、ALK4/ACVR1B、SKOR1、SKOR2、PRDM16、LEMD3、RBPMS、WWP1、RANBP3、PDPK1、DAB2 、USP15、PPP5C、LDLRAD4、PMEPA1、ZFHX3、ZNF451、SMURF2、PPM1A、TGF-β、TGFBR1、TGIF、TRIM33、ZNF580、NEDD4L、HGS、AIP1、WWP1、PML、ZNF8、RNF111 和 YAP1，参与多种细胞过程，包括信号转导、转录调控和蛋白质-蛋白质相互作用。

Human

E. coli

N-SUMO;N-6*His

Q15796 (S2-S467)

4087  [NCBI]

SSILPFTPPVVKRLLGWKKSAGGSGGAGGGEQNGQEEKWCEKAVKSLVKKLKKTGRLDELEKAITTQNCNTKCVTIPSTCSEIWGLSTPNTIDQWDTTGLYSFSEQTRSLDGRLQVSHRKGLPHVIYCRLWRWPDLHSHHELKAIENCEYAFNLKKDEVCVNPYHYQRVETPVLPPVLVPRHTEILTELPPLDDYTHSIPENTNFPAGIEPQSNYIPETPPPGYISEDGETSDQQLNQSMDTGSPAELSPTTLSPVNHSLDLQPVTYSEPAFWCSIAYYELNQRVGETFHASQPSLTVDGFTDPSNSERFCLGLLSNVNRNATVEMTRRHIGRGVRLYYIGGEVFAECLSDSAIFVQSPNCNQRYGWHPATVCKIPPGCNLKIFNNQEFAALLAQSVNQGFEAVYQLTRMCTIRMSFVKGWGAEYRRQTVTSTPCWIELHLNGPLQWLDKVLTQMGSPSVRCSSMS

Approximately 68.2 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.