SMPD1 蛋白, Human (sf9, His)

SMPD1 protein converts sphingomyelin to ceramide in lysosomes and has phospholipase C activity. SMPD1 Protein, Human (sf9, His) is the recombinant human-derived SMPD1 protein, expressed by Sf9 insect cells , with C-His labeled tag.

SMPD1 是一种多方面的酶，在鞘磷脂动态转化为神经酰胺方面发挥着重要作用，这一过程对于多种细胞功能至关重要。这种转化发生在溶酶体中，其中 SMPD1 在鞘脂代谢中发挥着关键作用。值得注意的是，SMPD1 表现出两种不同的酶促形式，这是由单一蛋白质前体的替代运输产生的，其中一种形式靶向内溶酶体区室，另一种形式释放到细胞外。在各种应激条件下，溶酶体胞吐作用成为分泌形式的主要来源，凸显了 SMPD1 对细胞信号的适应性。除了其鞘磷脂酶活性外，SMPD1 对内溶酶体内膜的胆固醇输出也有显着贡献，表明其参与脂质稳态。此外，SMPD1 对特定底物表现出磷脂酶 C 活性，强调了其在脂质代谢中的多功能性。除了脂质加工之外，SMPD1 通过调节神经酰胺的产生来对应激诱导的细胞凋亡进行调节，从而为其在细胞过程中的多功能作用增加了一层复杂性。

Measured by its ability to cleave 2-N-Hexadecanoylamino-4-nitrophenylphosphorylcholine (HNPPC). The specific activity is ≥1735 pmol/min/μg, as measured under the described conditions.

Human

Sf9 insect cells

C-His

P17405 (M1-P628)

6609  [NCBI]

Partial

Approximately 66.3 kDa.

Greater than 95% as determined by reducing SDS-PAGE.

溶液

Supplied as a 0.2 μm filtered solution of 20 mM Tris, 500 mM NaCl, 25 % glycerol, pH 7.5.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.