SOCS3 蛋白, Human (His-Trx)

SOCS3 is a member of the SOCS family that operates in a negative feedback loop to regulate cytokine signaling, particularly in the JAK/STAT pathway. It inhibits cytokine signaling by binding to receptors such as IL6ST/gp130, LIF, erythropoietin, insulin, IL12, GCSF, and leptin receptors. SOCS3 Protein, Human (His-Trx) is the recombinant human-derived SOCS3 protein, expressed by E. coli , with N-Trx, N-His labeled tag. The total length of SOCS3 Protein, Human (His-Trx) is 225 a.a., with molecular weight of ~46 kDa.

SOCS3 是 SOCS 家族的成员，在经典的负反馈系统中运作，该系统复杂地调节细胞因子信号转导。特别是，SOCS3对JAK/STAT通路发挥影响，参与细胞因子的负调节。通过与酪氨酸激酶受体（如 IL6ST/gp130、LIF、促红细胞生成素、胰岛素、IL12、GCSF 和瘦素受体）结合，SOCS3 有效抑制细胞因子信号转导。值得注意的是，它与 JAK2 的相互作用阻碍了后者的激酶活性，从而调节 IL6 信号传导。除了在信号转导中的作用之外，SOCS3 在抑制胎儿肝脏红细胞生成以及调节 2 型辅助 T 细胞介导的过敏反应的启动和维持方面也发挥着关键作用。此外，它还可以作为 SCF 样 ECS（Elongin BC-CUL2/5-SOCS-box 蛋白）E3 泛素-蛋白连接酶复合物中可能的底物识别组件，促进靶蛋白的泛素化和随后的蛋白酶体降解（这是一个重要方面）蛋白质修饰和泛素化过程。

Human

E. coli

N-Trx;N-His

O14543 (M1-L225)

9021  [NCBI]

Approximately 46 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 50 mM Tris, pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.