1. 重组蛋白
  2. Enzymes & Regulators
  3. Hydrolases (EC 3)
  4. srtA 蛋白, S. aureus

srtA 蛋白, S. aureus 是重组的 srtA 蛋白,由 E. coli 表达,不带标签。

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SPR 检测服务   重组蛋白定制服务

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2.  同一机构(单位)同一产品试用装仅限申领一次,同一机构(单位)一年内

     可免费申领三个不同产品的试用装。

3.  试用装只面向终端客户

规格 价格 是否有货 数量
10 μg ¥1500 1 - 2 周
50 μg ¥4200 1 - 2 周
100 μg   询价  

* Please select Quantity before adding items.

  • 生物活性

  • 技术参数

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  • 产品资料

描述

srtA Protein, S. aureus is the recombinant srtA protein, expressed by E. coli, with tag free tag.

研究背景

srtA 是一种转肽酶 (Transpeptidase),负责将表面蛋白锚定到细胞壁。它通过蛋白水解切割 C 端分选信号 (sorting signal) 来识别和修饰底物,随后通过硫酯键 (thioester bond) 形成 sortase 与底物之间的共价中间体,最终将其转移并共价连接到细胞壁。该酶识别 Leu-Pro-x-Thr-Gly (LPXTG) 基序,并在苏氨酸 (threonine) 和甘氨酸 (glycine) 残基之间切割。它利用 lipid II 作为肽聚糖 (peptidoglycan) 底物进行分选反应,并负责展示重要的毒力因子 (virulence factors),在感染过程中对宿主相互作用和定植起关键作用。by similar: 该功能在多个文献中被一致报道。

种属

Others

表达系统

E. coli

标签

Tag Free

蛋白编号

Q2FV99 (Q60-K206, P94S, D160N, D165A, G167E, K196T)

基因 ID

3921273

蛋白长度

Partial

中文名
sortaseA 蛋白
同用名
Sortase A; Surface protein sorting A; srtA; Staphylococcus aureus (strain NCTC 8325 / PS 47); Hydrolase; Protease; Thiol protease; 3.4.22.70
预测分子量
17 kDa
纯度

Greater than 90% as determined by reducing SDS-PAGE

性状

溶液.

组分

Supplied as a 0.22 μm filtered solution of 50 mM HEPES (pH 8.0), 500mM NaCl, 5% glycerol.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

Please use rapid thawing with running water to thaw the protein.

保存条件 & 期限

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

运输条件

Shipping with dry ice.

文件资料

srtA Protein, S. aureus 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
srtA Protein, S. aureus
目录号:
HY-P703433
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