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  3. SPARC 蛋白, Rat (HEK293, His)

SPARC 蛋白, Rat (HEK293, His)

目录号: HY-P73420
COA 产品使用指南

SPARC 蛋白通过与细胞外基质和细胞因子的相互作用来调节细胞生长。它结合钙和铜,以及各种成分,如胶原蛋白类型、白蛋白、血小板反应蛋白、PDGF 和细胞膜。值得注意的是,SPARC有两个钙结合位点,包括一个以低亲和力结合5至8个Ca(2+)离子的酸性结构域,以及一个以高亲和力结合Ca(2+)离子的EF手环。SPARC 蛋白, Rat (HEK293, His) 是重组的 SPARC 蛋白,由 HEK293 表达,带有 C-His 标签。SPARC 蛋白, Rat (HEK293, His) 全长 284 个氨基酸,分子量约为 ~33.8-40 kDa。

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5 μg ¥460 In-stock
10 μg ¥800 In-stock
50 μg ¥2200 In-stock
100 μg ¥3750 In-stock
500 μg ¥13000 In-stock
1 mg ¥22000 In-stock
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描述

SPARC protein regulates cell growth through interactions with the extracellular matrix and cytokines. It binds calcium and copper, as well as various components like collagen types, albumin, thrombospondin, PDGF, and cell membranes. Notably, SPARC has two calcium binding sites, including an acidic domain binding 5 to 8 Ca(2+) ions with low affinity, and an EF-hand loop binding a Ca(2+) ion with high affinity. SPARC Protein, Rat (HEK293, His) is the recombinant rat-derived SPARC protein, expressed by HEK293 , with C-His labeled tag. The total length of SPARC Protein, Rat (HEK293, His) is 284 a.a., with molecular weight of ~33.8-40 kDa.

研究背景

SPARC 蛋白通过与细胞外基质和细胞因子相互作用来参与细胞生长的调节。它具有钙和铜以及各种成分(例如不同类型的胶原蛋白、白蛋白、血小板反应蛋白、PDGF 和细胞膜)的结合能力。值得注意的是,它拥有两个钙结合位点,包括一个以低亲和力结合 5 至 8 个 Ca(2+) 离子的酸性结构域,以及一个以高亲和力结合 Ca(2+) 离子的 EF 手环。

生物活性

Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 3.581μg/mL, corresponding to a specific activity is 2.793×102 U/mg.

  • Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED50 for this effect is typically 3.581 μg/mL, corresponding to a specific activity is 2.793×102 U/mg.
种属

Rat

表达系统

HEK293

标签

C-His

蛋白编号

P16975 (A18-I301)

基因 ID

24791  [NCBI]

蛋白结构
N-term
SPARC (A18-I301)
Accession # P16975
His
C-term
同用名
SPARC; BM-40; Osteonectin; ON; Secreted Protein Acidic and Rich in Cysteine
氨基酸序列

APQTEAAEEMVAEETVVEETGLPVGANPVQVEMGEFEEGAEETVEEVVAENPCQNHHCKHGKVCELDESNTPMCVCQDPTSCPAPIGEFEKVCSNDNKTFDSSCHFFATKCTLEGTKKGHKLHLDYIGPCKYIAPCLDSELTEFPLRMRDWLKNVLVTLYERDEGNNLLTEKQKLRVKKIHENEKRLEAGDHPVELLARDFEKNYNMYIFPVHWQFGQLDQHPIDGYLSHTELAPLRAPLIPMEHCTTRFFETCDLDNDKYIALEEWAGCFGIKEQDINKDLVI

分子量

Approximately 33.8-40 kDa

纯度
  • Greater than 90% as determined by reducing SDS-PAGE.
性状

无菌粉末

组分

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

内毒素含量

<1 EU/μg, determined by LAL method.

复溶方法

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

保存条件 & 期限

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

运输条件

Room temperature in continental US; may vary elsewhere.

文件资料

SPARC Protein, Rat (HEK293, His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • 复溶计算器

  • 重组蛋白稀释计算器

  • 比活力计算器

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SPARC Protein, Rat (HEK293, His)
目录号:
HY-P73420
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