SPARCL1 蛋白, Mouse (650a.a, HEK293, His)

The SPARCL1 protein has important functions such as calcium ion, collagen and extracellular matrix binding. It plays a role in synaptic membrane adhesion, primarily at glutamatergic synapses. SPARCL1 Protein, Mouse (650a.a, HEK293, His) is the recombinant mouse-derived SPARCL1 protein, expressed by HEK293 , with C-His, C-10*His labeled tag. The total length of SPARCL1 Protein, Mouse (650a.a, HEK293, His) is 634 a.a., with molecular weight of ~95-100 kDa.

SPARCL1 蛋白预计具有重要功能，包括钙离子结合活性、胶原蛋白结合活性和细胞外基质结合活性。它在突触膜粘附中发挥作用，主要位于谷氨酸能突触中。SPARCL1 的表达谱跨越多种结构，包括心血管系统、中枢神经系统、男性生殖腺或器官、呼吸系统和感觉器官。这种蛋白质的偏向表达，特别是在成人膀胱和皮质中升高，突出了它在这些组织中的重要性，表明它参与了各种生理过程。SPARCL1 跨物种的保守性，正如其直系同源对应物 SPARC like 1 在人类中所见，强调了其进化重要性和潜在的功能保守性。

Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED₅₀ for this effect is 0.8933 μg/mL, corresponding to a specific activity is 1.12×10³ units/mg.

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  Measured by its ability to inhibit the cell growth of Mv-1-Lu mink lung epithelial cells. The ED₅₀ for this effect is 0.8933 μg/mL, corresponding to a specific activity is 1.12×10³ units/mg.

Mouse

HEK293

C-His;C-10*His

EDL20231.1 (I17-F650)

13602  [NCBI]

IPTSTRFLSDHSNPTTATLVTPEDATVPIAGVEATADIENHPNDKAEKPSALNSEEETHEQSTEQDKTYSFEVDLKDEEDGDGDLSVDPTEGTLTLDLQEGTSEPQQKSLPENGDFPATVSTSYVDPNQRANITKGKESQEQPVSDSHQQPNESSKQTQDLKAEESQTQDPDIPNEEEEEEEEEEEEEEEEPEDIGAPSDNQEEGKEPLEEQPTSKWEGNREQSDDTLEESSQPTQISKTEKHQSEQGNQGQESDSEAEGEDKAAGSKEHIPHTEQQDQEGKAGLEAIGNQKDTDEKAVSTEPTDAAVVPRSHGGAGDNGGGDDSKHGAGDDYFIPSQEFLEAERMHSLSYYLKYGGGEETTTGESENRREAADNQEAKKAESSPNAEPSDEGNSREHSAGSCTNFQCKRGHICKTDPQGKPHCVCQDPETCPPAKILDQACGTDNQTYASSCHLFATKCRLEGTKKGHQLQLDYFGACKSIPACTDFEVAQFPLRMRDWLKNILMQLYEPNPKHGGYLNEKQRSKVKKIYLDEKRLLAGDHPIELLLRDFKKNYHMYVYPVHWQFNELDQHPADRILTHSELAPLRASLVPMEHCITRFFEECDPNKDKHITLKEWGHCFGIKEEDIDENLLF

Approximately 95-100 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.