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SPG3A/ATL1 蛋白, Human (sf9, GST)

目录号: HY-P77214
产品使用指南

SPG3A/ATL1蛋白是一种GTP酶,通过形成反式同聚体促进膜束缚,从而实现内质网(ER)膜的同型融合。它对内质网管状网络的生物发生和细胞结构组织的形成做出了至关重要的贡献。SPG3A/ATL1 蛋白, Human (sf9, GST) 是重组的 SPG3A/ATL1 蛋白,由 Sf9 insect cells 表达,带有 N-GST 标签。

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描述

The SPG3A/ATL1 protein is a GTPase that promotes membrane tethering by forming trans homomers, thereby achieving homotypic fusion of the endoplasmic reticulum (ER) membrane. It makes a crucial contribution to the biogenesis of the endoplasmic reticulum tubular network and shaping the structural organization of cells. SPG3A/ATL1 Protein, Human (sf9, GST) is the recombinant human-derived SPG3A/ATL1 protein, expressed by Sf9 insect cells , with N-GST labeled tag.

研究背景

SPG3A/ATL1 蛋白作为 GTP 酶发挥作用,通过形成反式同聚体在膜束缚中发挥关键作用,促进内质网 (ER) 膜的同型融合。它的参与延伸到内质网管状网络的生物发生,有助于这种细胞结构的复杂组织。除了其主要功能外,SPG3A/ATL1 还可能参与高尔基体生物发生和轴突发育的调节。在作为脱辅基蛋白的单体状态和GDP结合形式下,SPG3A/ATL1在与GTP结合时转变为同二聚体,展示了其不同状态之间的动态相互作用。该蛋白质与关键细胞成分(包括 MAP4K4、REEP5、RTN3、RTN4、SPAST、TMED2、REEP1、CPT1C 和 ARL6IP1)进行各种相互作用,突出显示其参与复杂的分子关联网络。此外,SPG3A/ATL1 与 ZFYVE27 相互作用,进一步扩大其监管范围。

种属

Human

表达系统

Sf9 insect cells

标签

N-GST

蛋白编号

Q8WXF7-1 (M1-T447)

基因 ID
蛋白结构
N-term
GST
ATL1 (M1-T447)
Accession # Q8WXF7-1
C-term
同用名
Atlastin-1; GTP-binding protein 3; GBP-3; ATL1; SPG3A
分子量

Approximately 66 kDa

纯度

Greater than 80% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SPG3A/ATL1 Protein, Human (sf9, GST) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SPG3A/ATL1 Protein, Human (sf9, GST)
目录号:
HY-P77214
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