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  3. SPSB4 蛋白, Human (His)

SPSB4 蛋白, Human (His) 是重组的 SPSB4 蛋白,由 E. coli 表达,带有 His 标签。SPSB4 蛋白, Human (His)

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描述

SPSB4 Protein, Human (His) is the recombinant human-derived SPSB4, expressed by E. coli , with His labeled tag. ,

研究背景

SPSB4 是 SCF 样 ECS(Elongin BC-CUL2/5-SOCS-box 蛋白)E3 泛素-蛋白连接酶复合物的底物识别组分,介导靶蛋白的泛素化和随后的蛋白酶体降解(by similar)。它通过介导 NOS2 的泛素化和蛋白酶体降解,负调节一氧化氮(NO)的产生并限制活化巨噬细胞中的细胞毒性(by similar)。SPSB4 作为桥梁将 NOS2 与 ECS E3 连接酶复合物组分 ELOC 和 CUL5 连接起来(by similar)。它还通过介导 EphB2/CTF2 的泛素化和降解来减弱 EphB2 依赖性细胞排斥反应(by similar),并通过介导昼夜节律转录抑制因子 NR1D1 的泛素/蛋白酶体依赖性降解来调节细胞时钟功能。

种属

Human

表达系统

E. coli

标签

His

蛋白编号

Q96A44 (R33-E233)

基因 ID

92369

蛋白长度

Partial

中文名
SPSB4 蛋白
同用名
SSB4
预测分子量
23.7 kDa
纯度

Greater than 80% as determined by reducing SDS-PAGE

内毒素含量

<1 EU/μg, determined by LAL method.

文件资料

SPSB4 Protein, Human (His) 相关分类

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start) × 体积 (start) = 浓度 (final) × 体积 (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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产品名称:
SPSB4 Protein, Human (His)
目录号:
HY-P703378
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