SSTR2 Protein-VLP, Human (HEK293, His)

SSTR2 Protein-VLP, Human (HEK293, His) is recommended for animal immunization, ELISA, FACS. It is not recommended for receptor-ligand interaction detection and SPR/BLI assay since there are other irrelevant membrane proteins of the host on the VLP envelope, and the receptor-ligand interaction will have strong background interference. High requirements for chips and experimental protocols are needed for SPR/BLI assays. If VLP control is required, it is recommended HY-P701236. Tags can only be detected under denaturing conditions.

SSTR2 蛋白-VLP 作为生长抑素-14 和-28 的受体，与百日咳毒素敏感的 G 蛋白偶联以抑制腺苷酸环化酶。此外，它还通过对百日咳毒素不敏感和敏感的 G 蛋白刺激磷酸酪氨酸磷酸酶和 PLC，同时通过抑制电压依赖性钙通道来抑制钙进入。在胰腺 α 和 β 细胞中，SSTR2 作为功能主导的生长抑素受体，介导生长抑素-14 对激素分泌的抑制作用。除了在胰腺细胞中发挥作用外，SSTR2 还通过增强 MAPK1 和 MAPK2 磷酸化来抑制细胞生长，从而导致 CDKN1B 上调。它进一步有助于神经元迁移和轴突生长，可能参与大脑发育过程中的神经元发育和成熟。SSTR2 通过 PTPN6 介导胰岛素受体信号传导的负调节，并在异二聚化后使 SSTR3 受体功能失活。SSTR2 以同二聚体和异二聚体的形式与 SSTR3 和 SSTR5 一起存在，会经历激动剂诱导的解离成单体，这是受体内化的关键步骤。与 β-arrestin 和 SHANK1 的相互作用进一步调节受体内化和功能。

Measured by its binding ability in a functional ELISA. Immobilized Human SSTR2 at 1 μg/well can bind Anti-SSTR2 recombinant antibod, the EC₅₀is 70.87 - 130.6 ng/mL.

Human

HEK293

C-6*His

P30874 (M1-I369)

6752  [NCBI]

Full Length

MDMADEPLNGSHTWLSIPFDLNGSVVSTNTSNQTEPYYDLTSNAVLTFIYFVVCIIGLCGNTLVIYVILRYAKMKTITNIYILNLAIADELFMLGLPFLAMQVALVHWPFGKAICRVVMTVDGINQFTSIFCLTVMSIDRYLAVVHPIKSAKWRRPRTAKMITMAVWGVSLLVILPIMIYAGLRSNQWGRSSCTINWPGESGAWYTGFIIYTFILGFLVPLTIICLCYLFIIIKVKSSGIRVGSSKRKKSEKKVTRMVSIVVAVFIFCWLPFYIFNVSSVSMAISPTPALKGMFDFVVVLTYANSCANPILYAFLSDNFKKSFQNVLCLVKVSGTDDGERSDSKQDKSRLNETTETQRTLLNGDLQTSI

42.5 kDa

无菌粉末.

Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.