STK11 蛋白, Human (His-SUMO)

The STK11 protein is a tumor suppressor kinase that complexly regulates members of the AMP-activated protein kinase (AMPK) family, affecting cellular processes such as metabolism, apoptosis, and DNA damage responses. It phosphorylates the T-loop of AMPK family proteins and activates PRKAA1, PRKAA2 and other proteins (except MELK). STK11 Protein, Human (His-SUMO) is the recombinant human-derived STK11 protein, expressed by E. coli , with N-SUMO, N-6*His labeled tag. The total length of STK11 Protein, Human (His-SUMO) is 430 a.a., with molecular weight (affected by relative charge) of ~72 KDa.

STK11 蛋白是一种肿瘤抑制丝氨酸/苏氨酸蛋白激酶，复杂地调节 AMP 激活蛋白激酶 (AMPK) 家族成员的活性，对多种细胞过程产生影响，包括代谢、细胞极性、细胞凋亡和 DNA 损伤反应。STK11 通过 AMPK 家族蛋白（如 PRKAA1、PRKAA2、BRSK1、BRSK2、MARK1、MARK2、MARK3、MARK4、NUAK1、NUAK2、SIK1、SIK2、SIK3 和 SNRK）T 环的磷酸化来促进其激活，同时梅尔克除外。除了 AMPK 家族之外，STK11 将其调控范围扩展到非 AMPK 蛋白，例如 STRADA、PTEN，可能还有 p53/TP53。作为 AMPK 的关键上游调节因子，STK11 协调细胞反应，包括在低能量条件下抑制生长促进信号通路、维持肝脏中的葡萄糖稳态、在营养缺乏的细胞中启动自噬以及促进 B 细胞生发中心响应 DNA 损伤而分化。此外，STK11 通过重塑肌动蛋白细胞骨架在细胞极性中发挥着至关重要的作用，并且通过 BRSK1 和 BRSK2 的磷酸化和激活对于皮质神经元极化至关重要。在 DNA 损伤反应领域，STK11 与 p53/TP53 相互作用，参与 CDKN1A/WAF1 启动子上的转录激活，并充当 p53/TP53 依赖性细胞凋亡的介质。此外，STK11 与 NUAK1 协同磷酸化 CDKN1A，从而参与紫外线辐射诱导的 DNA 损伤反应，有助于其降解以实现最佳 DNA 修复，并且它还在精子发生中发挥作用。

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-SUMO;N-6*His

Q15831 (M1-C430)

6794  [NCBI]

MEVVDPQQLGMFTEGELMSVGMDTFIHRIDSTEVIYQPRRKRAKLIGKYLMGDLLGEGSYGKVKEVLDSETLCRRAVKILKKKKLRRIPNGEANVKKEIQLLRRLRHKNVIQLVDVLYNEEKQKMYMVMEYCVCGMQEMLDSVPEKRFPVCQAHGYFCQLIDGLEYLHSQGIVHKDIKPGNLLLTTGGTLKISDLGVAEALHPFAADDTCRTSQGSPAFQPPEIANGLDTFSGFKVDIWSAGVTLYNITTGLYPFEGDNIYKLFENIGKGSYAIPGDCGPPLSDLLKGMLEYEPAKRFSIRQIRQHSWFRKKHPPAEAPVPIPPSPDTKDRWRSMTVVPYLEDLHGADEDEDLFDIEDDIIYTQDFTVPGQVPEEEASHNGQRRGLPKAVCMNGTEAAQLSTKSRAEGRAPNPARKACSASSKIRRLSAC

Approximately 72 kDa. The reducing (R) protein migrat es as 72 kDa in SDS-PAGE may be due to relative charge.

Greater than 90% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm sterile filtered 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O.

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.