Stratifin 蛋白, Human

Stratifin (14-3-3 sigma), encoded by SFN, functions as a multifaceted adapter protein, engaging in diverse cellular processes by binding to partners via phosphoserine or phosphothreonine motifs. It regulates epithelial cell growth and protein synthesis with keratin 17 (KRT17) and potentially influences MDM2 autoubiquitination, activating p53. As a homodimer, it interacts in various complexes with proteins like GAB2, SAMSN1, SRPK2, COPS6, COP1, DAPK2, PI4KB, SLITRK1, and LRRK2, highlighting its versatile role in cellular processes and signaling pathways. Stratifin Protein, Human is the recombinant mouse-derived Stratifin protein, expressed by E. coli, with tag free. The total length of Stratifin Protein, Human is 248 a.a., with molecular weight of ~30.0 kDa.

Stratifin，也称为14-3-3 sigma，由SFN基因编码，是属于14-3-3家族的多功能蛋白。它作为衔接蛋白，通过识别磷酸丝氨酸或磷酸苏氨酸基序与众多伙伴结合，参与多种细胞过程。其关键作用包括与角蛋白 17 (KRT17) 结合时调节上皮细胞生长和蛋白质合成，以及潜在参与 MDM2 自动泛素化和降解，从而导致肿瘤抑制因子 p53 的激活。stratifin 以同二聚体形式存在并参与各种蛋白质复合物，可与多种蛋白质相互作用，例如 GAB2、SAMSN1、SRPK2、COPS6、COP1、DAPK2、PI4KB、SLITRK1 和 LRRK2，展示了其在协调关键细胞过程和信号通路。

Mouse

E. coli

Tag Free

P31947 (M1-S248)

2810  [NCBI]

MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS

Approximately 30.0 kDa

Greater than 95% as determined by reducing SDS-PAGE.

无菌粉末.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, 1 mM EDTA, 1 mM DTT, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US;may vary elsewhere.